5L24

Structure of CNTnw N149L in the intermediate 2 state

5L24 の概要

• エントリーDOI: 10.2210/pdb5l24/pdb
• 関連するPDBエントリー: 5L26 5L27 5L28 5L29 5L2A 5L2B
• 分子名称: Nucleoside permease, 2-{[(4-O-alpha-D-glucopyranosyl-beta-D-glucopyranosyl)oxy]methyl}-2-octyldecyl 4-O-alpha-D-glucopyranosyl-beta-D-glucopyranoside (2 entities in total)
• 機能のキーワード: transporter, nucleoside, elevator-type alternate access, transport protein
• 由来する生物種: Neisseria wadsworthii 9715
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 135918.97

構造登録者

Hirschi, M.,Johnson, Z.L.,Lee, S.-Y. (登録日: 2016-07-31, 公開日: 2017-04-12, 最終更新日: 2023-10-04)

主引用文献

Hirschi, M.,Johnson, Z.L.,Lee, S.Y.
Visualizing multistep elevator-like transitions of a nucleoside transporter.
Nature, 545:66-70, 2017

PubMed Abstract: Membrane transporters move substrates across the membrane by alternating access of their binding sites between the opposite sides of the membrane. An emerging model of this process is the elevator mechanism, in which a substrate-binding transport domain moves a large distance across the membrane. This mechanism has been characterized by a transition between two states, but the conformational path that leads to the transition is not yet known, largely because the available structural information has been limited to the two end states. Here we present crystal structures of the inward-facing, intermediate, and outward-facing states of a concentrative nucleoside transporter from Neisseria wadsworthii. Notably, we determined the structures of multiple intermediate conformations, in which the transport domain is captured halfway through its elevator motion. Our structures present a trajectory of the conformational transition in the elevator model, revealing multiple intermediate steps and state-dependent conformational changes within the transport domain that are associated with the elevator-like motion.

PubMed: 28424521
DOI: 10.1038/nature22057

実験手法

X-RAY DIFFRACTION (4.1 Å)