5KYW

crystal structure of Sec23 and TANGO1 peptide3 complex

5KYW の概要

• エントリーDOI: 10.2210/pdb5kyw/pdb
• 分子名称: Protein transport protein Sec23A, Protein transport protein Sec24D, TANGO1 peptide3, ... (4 entities in total)
• 機能のキーワード: copii coat, collagen secretion, cargo adapter, vesicle, protein transport
• 由来する生物種: Homo sapiens (Human); 詳細
• 細胞内の位置: Smooth endoplasmic reticulum membrane; Peripheral membrane protein: Q15436; Cytoplasm : O94855
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 173940.27

構造登録者

Ma, W.,Goldberg, J. (登録日: 2016-07-22, 公開日: 2016-09-07, 最終更新日: 2024-11-20)

主引用文献

Ma, W.,Goldberg, J.
TANGO1/cTAGE5 receptor as a polyvalent template for assembly of large COPII coats.
Proc.Natl.Acad.Sci.USA, 113:10061-10066, 2016

PubMed Abstract: The supramolecular cargo procollagen is loaded into coat protein complex II (COPII)-coated carriers at endoplasmic reticulum (ER) exit sites by the receptor molecule TANGO1/cTAGE5. Electron microscopy studies have identified a tubular carrier of suitable dimensions that is molded by a distinctive helical array of the COPII inner coat protein Sec23/24•Sar1; the helical arrangement is absent from canonical COPII-coated small vesicles. In this study, we combined X-ray crystallographic and biochemical analysis to characterize the association of TANGO1/cTAGE5 with COPII proteins. The affinity for Sec23 is concentrated in the proline-rich domains (PRDs) of TANGO1 and cTAGE5, but Sec23 recognizes merely a PPP motif. The PRDs contain repeated PPP motifs separated by proline-rich linkers, so a single TANGO1/cTAGE5 receptor can bind multiple copies of coat protein in a close-packed array. We propose that TANGO1/cTAGE5 promotes the accretion of inner coat proteins to the helical lattice. Furthermore, we show that PPP motifs in the outer coat protein Sec31 also bind to Sec23, suggesting that stepwise COPII coat assembly will ultimately displace TANGO1/cTAGE5 and compartmentalize its operation to the base of the growing COPII tubule.

PubMed: 27551091
DOI: 10.1073/pnas.1605916113

実験手法

X-RAY DIFFRACTION (3.2 Å)