5KUC

Crystal structure of trypsin activated Cry6Aa

「5J65」から置き換えられました

5KUC の概要

• エントリーDOI: 10.2210/pdb5kuc/pdb
• 関連するPDBエントリー: 5KUD
• 分子名称: Pesticidal crystal protein Cry6Aa (2 entities in total)
• 機能のキーワード: pesticidal, pore formation, toxin
• 由来する生物種: Bacillus thuringiensis
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 54131.22

構造登録者

Kelker, M.S.,Xu, X.,Lee, M.,Chan, M.,Hung, S.,Dementiev, K.,Hey, T.,Chikwana, V.M.,Narva, K.E. (登録日: 2016-07-13, 公開日: 2016-08-03, 最終更新日: 2024-10-09)

主引用文献

Dementiev, A.,Board, J.,Sitaram, A.,Hey, T.,Kelker, M.S.,Xu, X.,Hu, Y.,Vidal-Quist, C.,Chikwana, V.,Griffin, S.,McCaskill, D.,Wang, N.X.,Hung, S.C.,Chan, M.K.,Lee, M.M.,Hughes, J.,Wegener, A.,Aroian, R.V.,Narva, K.E.,Berry, C.
The pesticidal Cry6Aa toxin from Bacillus thuringiensis is structurally similar to HlyE-family alpha pore-forming toxins.
Bmc Biol., 14:71-71, 2016

PubMed Abstract: The Cry6 family of proteins from Bacillus thuringiensis represents a group of powerful toxins with great potential for use in the control of coleopteran insects and of nematode parasites of importance to agriculture. These proteins are unrelated to other insecticidal toxins at the level of their primary sequences and the structure and function of these proteins has been poorly studied to date. This has inhibited our understanding of these toxins and their mode of action, along with our ability to manipulate the proteins to alter their activity to our advantage. To increase our understanding of their mode of action and to facilitate further development of these proteins we have determined the structure of Cry6Aa in protoxin and trypsin-activated forms and demonstrated a pore-forming mechanism of action.

PubMed: 27576487
DOI: 10.1186/s12915-016-0295-9

実験手法

X-RAY DIFFRACTION (2 Å)