5KTL

Dihydrodipicolinate synthase from the industrial and evolutionarily important cyanobacteria Anabaena variabilis.

5KTL の概要

• エントリーDOI: 10.2210/pdb5ktl/pdb
• 関連するPDBエントリー: 5KT0
• 分子名称: 4-hydroxy-tetrahydrodipicolinate synthase, MANGANESE (II) ION (3 entities in total)
• 機能のキーワード: dhdps, enzyme, cyanobacteria, diaminopimelate biosynthesis pathway, lyase
• 由来する生物種: Anabaena variabilis
• 細胞内の位置: Cytoplasm : Q3M723
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 71270.78

構造登録者

Christensen, J.B.,Panjikar, S.,Perugini, M. (登録日: 2016-07-11, 公開日: 2016-11-30, 最終更新日: 2025-04-02)

主引用文献

Christensen, J.B.,Soares da Costa, T.P.,Faou, P.,Pearce, F.G.,Panjikar, S.,Perugini, M.A.
Structure and Function of Cyanobacterial DHDPS and DHDPR.
Sci Rep, 6:37111-37111, 2016

PubMed Abstract: Lysine biosynthesis in bacteria and plants commences with a condensation reaction catalysed by dihydrodipicolinate synthase (DHDPS) followed by a reduction reaction catalysed by dihydrodipicolinate reductase (DHDPR). Interestingly, both DHDPS and DHDPR exist as different oligomeric forms in bacteria and plants. DHDPS is primarily a homotetramer in all species, but the architecture of the tetramer differs across kingdoms. DHDPR also exists as a tetramer in bacteria, but has recently been reported to be dimeric in plants. This study aimed to characterise for the first time the structure and function of DHDPS and DHDPR from cyanobacteria, which is an evolutionary important phylum that evolved at the divergence point between bacteria and plants. We cloned, expressed and purified DHDPS and DHDPR from the cyanobacterium Anabaena variabilis. The recombinant enzymes were shown to be folded by circular dichroism spectroscopy, enzymatically active employing the quantitative DHDPS-DHDPR coupled assay, and form tetramers in solution using analytical ultracentrifugation. Crystal structures of DHDPS and DHDPR from A. variabilis were determined at 1.92 Å and 2.83 Å, respectively, and show that both enzymes adopt the canonical bacterial tetrameric architecture. These studies indicate that the quaternary structure of bacterial and plant DHDPS and DHDPR diverged after cyanobacteria evolved.

PubMed: 27845445
DOI: 10.1038/srep37111

実験手法

X-RAY DIFFRACTION (1.92 Å)