5KQO

1-deoxy-D-xylulose 5-phosphate reductoisomerase from Vibrio vulnificus

5KQO の概要

• エントリーDOI: 10.2210/pdb5kqo/pdb
• 分子名称: 1-deoxy-D-xylulose 5-phosphate reductoisomerase, PHOSPHATE ION, 1,2-ETHANEDIOL, ... (4 entities in total)
• 機能のキーワード: dxr, 1-deoxy-d-xylulose 5-phosphate reductoisomerase, oxidoreductase
• 由来する生物種: Vibrio vulnificus (strain CMCP6)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 92894.75

構造登録者

Ussin, N.,Offermann, L.R.,Perdue, M.,Chruszcz, M. (登録日: 2016-07-06, 公開日: 2017-07-12, 最終更新日: 2023-10-04)

主引用文献

Ussin, N.K.,Bagnell, A.M.,Offermann, L.R.,Abdulsalam, R.,Perdue, M.L.,Magee, P.,Chruszcz, M.
Structural characterization of 1-deoxy-D-xylulose 5-phosphate Reductoisomerase from Vibrio vulnificus.
Biochim Biophys Acta Proteins Proteom, 1866:1209-1215, 2018

PubMed Abstract: Vibrio vulnificus, a gram-negative bacterium, is the leading cause of seafood-borne illnesses and mortality in the United States. Previous studies have identified metabolites 2-C-methylerythritol 4-phosphate (MEP) as being essential for V. vulnificus growth and function. It was shown that 1-deoxy-D-xylulose-5-phosphate reductoisomerase (Dxr) is a critical enzyme in the viability of V. vulnificus, and many other bacteria, as it catalyzes the rearrangement of 1-deoxy-D-xylulose-5-phosphate (Dxp) to 2-C-methylerythritol 4-phosphate (MEP) within the MEP pathway, found in plants and bacteria. The MEP pathway produces the isoprenoids, isopentenyl diphosphate and dimethylallyl pyrophosphate. In this study, we produced and structurally characterized V. vulnificus Dxr. The enzyme forms a dimeric assembly and contains a metal ion in the active site. Protein produced in Escherichia coli co-purifies with Mg ions, however the Mg cations may be substituted with Mn, as both of these metals may be utilized by Dxrs. These findings will provide a basis for the design of Dxr inhibitors that may find application as antimicrobial compounds.

PubMed: 30278288
DOI: 10.1016/j.bbapap.2018.09.008

実験手法

X-RAY DIFFRACTION (2.35 Å)