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5KNW

Solution NMR structure of human LARP7 xRRM2

5KNW の概要
エントリーDOI10.2210/pdb5knw/pdb
NMR情報BMRB: 30126
分子名称La-related protein 7 (1 entity in total)
機能のキーワードxrrm, rrm, 7sk, rna binding protein
由来する生物種Homo sapiens (Human)
タンパク質・核酸の鎖数1
化学式量合計14395.44
構造登録者
Eichhorn, C.D.,Feigon, J. (登録日: 2016-06-28, 公開日: 2016-10-12, 最終更新日: 2024-05-15)
主引用文献Eichhorn, C.D.,Chug, R.,Feigon, J.
hLARP7 C-terminal domain contains an xRRM that binds the 3' hairpin of 7SK RNA.
Nucleic Acids Res., 44:9977-9989, 2016
Cited by
PubMed Abstract: The 7SK small nuclear ribonucleoprotein (snRNP) sequesters and inactivates the positive transcription elongation factor b (P-TEFb), an essential eukaryotic mRNA transcription factor. The human La-related protein group 7 (hLARP7) is a constitutive component of the 7SK snRNP and localizes to the 3' terminus of the 7SK long noncoding RNA. hLARP7, and in particular its C-terminal domain (CTD), is essential for 7SK RNA stability and assembly with P-TEFb. The hLARP7 N-terminal La module binds and protects the 3' end from degradation, but the structural and functional role of its CTD is unclear. We report the solution NMR structure of the hLARP7 CTD and show that this domain contains an xRRM, a class of atypical RRM first identified in the Tetrahymena thermophila telomerase LARP7 protein p65. The xRRM binds the 3' end of 7SK RNA at the top of stem-loop 4 (SL4) and interacts with both unpaired and base-paired nucleotides. This study confirms that the xRRM is general to the LARP7 family of proteins and defines the binding site for hLARP7 on the 7SK RNA, providing insight into function.
PubMed: 27679474
DOI: 10.1093/nar/gkw833
主引用文献が同じPDBエントリー
実験手法
SOLUTION NMR
構造検証レポート
Validation report summary of 5knw
検証レポート(詳細版)ダウンロードをダウンロード

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件を2024-11-20に公開中

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