5KJ9

Anticancer activity of Ru- and Os(arene) compounds of a maleimide-functionalized bioactive pyridinecarbothioamide ligand

5KJ9 の概要

• エントリーDOI: 10.2210/pdb5kj9/pdb
• 分子名称: Lysozyme C, SODIUM ION, RUTHENIUM ION, ... (4 entities in total)
• 機能のキーワード: lysozyme, anticancer, ligand, ruthenium, hydrolase
• 由来する生物種: Gallus gallus (Chicken)
• 細胞内の位置: Secreted: P00698
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14455.22

構造登録者

Sullivan, M.P.,Christian, C.G.,Goldstone, D.C. (登録日: 2016-06-18, 公開日: 2016-07-06, 最終更新日: 2024-11-20)

主引用文献

Hanif, M.,Moon, S.,Sullivan, M.P.,Movassaghi, S.,Kubanik, M.,Goldstone, D.C.,Sohnel, T.,Jamieson, S.M.,Hartinger, C.G.
Anticancer activity of Ru- and Os(arene) compounds of a maleimide-functionalized bioactive pyridinecarbothioamide ligand.
J. Inorg. Biochem., 165:100-107, 2016

PubMed Abstract: With the aim of increasing the accumulation of Ru anticancer agents in the tumor, a targeted delivery strategy based on a maleimide anchor for the biological vector human serum albumin (HSA) was developed. A group of piano stool Ru- and Os(η-arene) complexes carrying a maleimide-functionalized N-phenyl-2-pyridinecarbothioamide (PCA) ligand was designed allowing for covalent conjugation to biological thiols. The complexes were characterized by NMR spectroscopy, ESI-MS, elemental analysis and single-crystal X-ray diffraction analysis. The compounds were shown to undergo halido/aqua ligand exchange reactions in aqueous solution, depending mainly on the metal center and the nature of the halide. In vitro cytotoxicity studies revealed low potency which is explained by the observed high reactivity of the maleimide to the thiol of l-cysteine (Cys), while the metal center itself shows little affinity to amino acids of the model protein lysozyme.

PubMed: 27470012
DOI: 10.1016/j.jinorgbio.2016.06.025

実験手法

X-RAY DIFFRACTION (1.2 Å)