5KE1

Structure of a C-terminal fragment of the IcsA/VirG passenger-domain

Summary for 5KE1

• Entry DOI: 10.2210/pdb5ke1/pdb
• Descriptor: Outer membrane protein IcsA autotransporter, NICKEL (II) ION (3 entities in total)
• Functional Keywords: virulence factor, autochaperone, autotransporter, transport protein
• Biological source: Shigella flexneri
• Cellular location: Outer membrane protein IcsA autotransporter: Periplasm. Outer membrane protein IcsA: Secreted. Outer membrane protein IcsA translocator: Cell outer membrane ; Multi-pass membrane protein : Q7BCK4
• Total number of polymer chains: 2
• Total formula weight: 73261.85

Authors

Leupold, S.,Scrima, A. (deposition date: 2016-06-09, release date: 2017-03-15, Last modification date: 2024-01-10)

Primary citation

Leupold, S.,Busing, P.,Mas, P.J.,Hart, D.J.,Scrima, A.
Structural insights into the architecture of the Shigella flexneri virulence factor IcsA/VirG and motifs involved in polar distribution and secretion.
J. Struct. Biol., 198:19-27, 2017

PubMed Abstract: IcsA/VirG is a key virulence factor of the human pathogen Shigella flexneri, acting as both an adhesin and actin-polymerizing factor during infection. We identified a soluble expression construct of the IcsA/VirG α-domain using the ESPRIT library screening system and determined its structure to 1.9Å resolution. In addition to the previously characterized autochaperone domain, our structure reveals a new domain, which shares a common fold with the autochaperone domains of various autotransporters. We further provide insight into the previously structurally uncharacterized β-helix domain that harbors the polar targeting motif and passenger-associated transport repeat. This structure is the first of any member of the recently identified passenger-associated transport repeat-containing autotransporters. Thus, it provides new insights into the overall architecture of this class of autotransporters, the function of the identified additional autochaperone domain and the structural properties of motifs involved in polar targeting and secretion of the Shigella flexneri virulence factor IcsA/VirG.

PubMed: 28268178
DOI: 10.1016/j.jsb.2017.03.003

Experimental method

X-RAY DIFFRACTION (1.9 Å)