NH3-bound RT XFEL structure of Photosystem II 500 ms after the 2nd illumination (2F) at 2.8 A resolution

Summary for 5KAI

Related5KAF 5TIS
DescriptorPhotosystem II protein D1 1, Photosystem II CP47 reaction center protein, Photosystem II CP43 reaction center protein, ... (35 entities in total)
Functional Keywordsphotosystems, transmembrane, room temperature, electron transport
Biological sourceThermosynechococcus elongatus (strain BP-1)
Total number of polymer chains40
Total molecular weight764162.27
Primary citation
Young, I.D.,Ibrahim, M.,Chatterjee, R.,Gul, S.,Fuller, F.D.,Koroidov, S.,Brewster, A.S.,Tran, R.,Alonso-Mori, R.,Kroll, T.,Michels-Clark, T.,Laksmono, H.,Sierra, R.G.,Stan, C.A.,Hussein, R.,Zhang, M.,Douthit, L.,Kubin, M.,de Lichtenberg, C.,Vo Pham, L.,Nilsson, H.,Cheah, M.H.,Shevela, D.,Saracini, C.,Bean, M.A.,Seuffert, I.,Sokaras, D.,Weng, T.C.,Pastor, E.,Weninger, C.,Fransson, T.,Lassalle, L.,Brauer, P.,Aller, P.,Docker, P.T.,Andi, B.,Orville, A.M.,Glownia, J.M.,Nelson, S.,Sikorski, M.,Zhu, D.,Hunter, M.S.,Lane, T.J.,Aquila, A.,Koglin, J.E.,Robinson, J.,Liang, M.,Boutet, S.,Lyubimov, A.Y.,Uervirojnangkoorn, M.,Moriarty, N.W.,Liebschner, D.,Afonine, P.V.,Waterman, D.G.,Evans, G.,Wernet, P.,Dobbek, H.,Weis, W.I.,Brunger, A.T.,Zwart, P.H.,Adams, P.D.,Zouni, A.,Messinger, J.,Bergmann, U.,Sauter, N.K.,Kern, J.,Yachandra, V.K.,Yano, J.
Structure of photosystem II and substrate binding at room temperature.
Nature, 540:453-457, 2016
PubMed: 27871088 (PDB entries with the same primary citation)
DOI: 10.1038/nature20161
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (2.80000914773 Å)

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers 0.3007 0.6% 0.9% 3.3%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution
Download full validation report