5K3W

Structural characterisation of fold IV-transaminase, CpuTA1, from Curtobacterium pusillum

5K3W の概要

• エントリーDOI: 10.2210/pdb5k3w/pdb
• 分子名称: CpuTA1, PYRIDOXAL-5'-PHOSPHATE, 3-AMINOBENZOIC ACID, ... (4 entities in total)
• 機能のキーワード: transaminase, curtobacterium pusillum, transferase
• 由来する生物種: Curtobacterium pusillum
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 68166.15

構造登録者

Pavkov-Keller, T.,Diepold, M.,Steiner, K.,Gruber, K. (登録日: 2016-05-20, 公開日: 2016-12-14, 最終更新日: 2024-01-10)

主引用文献

Pavkov-Keller, T.,Strohmeier, G.A.,Diepold, M.,Peeters, W.,Smeets, N.,Schurmann, M.,Gruber, K.,Schwab, H.,Steiner, K.
Discovery and structural characterisation of new fold type IV-transaminases exemplify the diversity of this enzyme fold.
Sci Rep, 6:38183-38183, 2016

PubMed Abstract: Transaminases are useful biocatalysts for the production of amino acids and chiral amines as intermediates for a broad range of drugs and fine chemicals. Here, we describe the discovery and characterisation of new transaminases from microorganisms which were enriched in selective media containing (R)-amines as sole nitrogen source. While most of the candidate proteins were clearly assigned to known subgroups of the fold IV family of PLP-dependent enzymes by sequence analysis and characterisation of their substrate specificity, some of them did not fit to any of these groups. The structure of one of these enzymes from Curtobacterium pusillum, which can convert d-amino acids and various (R)-amines with high enantioselectivity, was solved at a resolution of 2.4 Å. It shows significant differences especially in the active site compared to other transaminases of the fold IV family and thus indicates the existence of a new subgroup within this family. Although the discovered transaminases were not able to convert ketones in a reasonable time frame, overall, the enrichment-based approach was successful, as we identified two amine transaminases, which convert (R)-amines with high enantioselectivity, and can be used for a kinetic resolution of 1-phenylethylamine and analogues to obtain the (S)-amines with e.e.s >99%.

PubMed: 27905516
DOI: 10.1038/srep38183

実験手法

X-RAY DIFFRACTION (2.503 Å)