5K2J

Crystal structure of reduced Prx3 in complex with h2o2 from Vibrio vulnificus

5K2J の概要

• エントリーDOI: 10.2210/pdb5k2j/pdb
• 関連するPDBエントリー: 5K1G 5K2I
• 分子名称: 1-Cys peroxiredoxin, HYDROGEN PEROXIDE (3 entities in total)
• 機能のキーワード: 1-cys prx, vibrio vulnificus, hydrogen peroxide, nitric oxide, oxidoreductase
• 由来する生物種: Vibrio vulnificus MO6-24/O
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 210687.71

構造登録者

Ahn, J.,Ha, N.-C. (登録日: 2016-05-19, 公開日: 2017-05-24, 最終更新日: 2023-11-08)

主引用文献

Ahn, J.,Jang, K.K.,Jo, I.,Nurhasni, H.,Lim, J.G.,Yoo, J.W.,Choi, S.H.,Ha, N.C.
Crystal structure of peroxiredoxin 3 fromVibrio vulnificusand its implications for scavenging peroxides and nitric oxide.
IUCrJ, 5:82-92, 2018

PubMed Abstract: Peroxiredoxins (Prxs) are ubiquitous cysteine-based peroxidase enzymes. Recently, a new type of Prx, Prx3, was identified in the pathogenic bacterium as being important for survival in macrophages. It employs only one catalytic cysteine residue to decompose peroxides. Here, crystal structures of Prx3 representing its reduced and oxidized states have been determined, together with an HO-bound structure, at high resolution. The crystal structure representing the reduced Prx3 showed a typical dimeric interface, called the A-type interface. However, Prx3 forms an oligomeric interface mediated by a disulfide bond between two catalytic cysteine residues from two adjacent dimers, which differs from the doughnut-like oligomers that appear in most Prxs. Subsequent biochemical studies showed that this disulfide bond was induced by treatment with nitric oxide (NO) as well as with peroxides. Consistently, NO treatment induced expression of the gene in , and Prx3 was crucial for the survival of bacteria in the presence of NO. Taken together, the function and mechanism of Prx3 in scavenging peroxides and NO stress oligomerization are proposed. These findings contribute to the understanding of the diverse functions of Prxs during pathogenic processes at the molecular level.

PubMed: 29354274
DOI: 10.1107/S205225251701750X

実験手法

X-RAY DIFFRACTION (1.908 Å)