5K2F

Structure of NNQQNY from yeast prion Sup35 with cadmium acetate determined by MicroED

5K2F の概要

• エントリーDOI: 10.2210/pdb5k2f/pdb
• 関連するPDBエントリー: 5K2E 5K2G 5K2H
• EMDBエントリー: 8196 8197 8198 8199
• 分子名称: Eukaryotic peptide chain release factor GTP-binding subunit, CADMIUM ION, ACETATE ION, ... (4 entities in total)
• 機能のキーワード: amyloid, yeast prion, protein fibril
• 由来する生物種: Saccharomyces cerevisiae (Baker's yeast)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 951.21

構造登録者

Rodriguez, J.A.,Sawaya, M.R.,Cascio, D.,Eisenberg, D.S. (登録日: 2016-05-18, 公開日: 2016-09-14, 最終更新日: 2024-03-06)

主引用文献

Sawaya, M.R.,Rodriguez, J.,Cascio, D.,Collazo, M.J.,Shi, D.,Reyes, F.E.,Hattne, J.,Gonen, T.,Eisenberg, D.S.
Ab initio structure determination from prion nanocrystals at atomic resolution by MicroED.
Proc.Natl.Acad.Sci.USA, 113:11232-11236, 2016

PubMed Abstract: Electrons, because of their strong interaction with matter, produce high-resolution diffraction patterns from tiny 3D crystals only a few hundred nanometers thick in a frozen-hydrated state. This discovery offers the prospect of facile structure determination of complex biological macromolecules, which cannot be coaxed to form crystals large enough for conventional crystallography or cannot easily be produced in sufficient quantities. Two potential obstacles stand in the way. The first is a phenomenon known as dynamical scattering, in which multiple scattering events scramble the recorded electron diffraction intensities so that they are no longer informative of the crystallized molecule. The second obstacle is the lack of a proven means of de novo phase determination, as is required if the molecule crystallized is insufficiently similar to one that has been previously determined. We show with four structures of the amyloid core of the Sup35 prion protein that, if the diffraction resolution is high enough, sufficiently accurate phases can be obtained by direct methods with the cryo-EM method microelectron diffraction (MicroED), just as in X-ray diffraction. The success of these four experiments dispels the concern that dynamical scattering is an obstacle to ab initio phasing by MicroED and suggests that structures of novel macromolecules can also be determined by direct methods.

PubMed: 27647903
DOI: 10.1073/pnas.1606287113

実験手法

ELECTRON CRYSTALLOGRAPHY (1 Å)