5K1Q

Crystal structure of oxidized Shewanella Yellow Enzyme 4 (SYE4) in complex with p-methylphenol

5K1Q の概要

• エントリーDOI: 10.2210/pdb5k1q/pdb
• 分子名称: NAD(P)H:flavin oxidoreductase Sye4, FLAVIN MONONUCLEOTIDE, P-CRESOL, ... (4 entities in total)
• 機能のキーワード: oxidoreductase, cofactor-binding
• 由来する生物種: Shewanella oneidensis (strain MR-1)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 40234.21

構造登録者

Elegheert, J.,Brige, A.,Savvides, S.N. (登録日: 2016-05-18, 公開日: 2017-06-07, 最終更新日: 2024-01-10)

主引用文献

Elegheert, J.,Brige, A.,Van Beeumen, J.,Savvides, S.N.
Structural dissection of Shewanella oneidensis old yellow enzyme 4 bound to a Meisenheimer complex and (nitro)phenolic ligands.
FEBS Lett., 591:3391-3401, 2017

PubMed Abstract: Shewanella oneidensis, a Gram-negative γ-proteobacterium with an extensive redox capacity, possesses four old yellow enzyme (OYE) homologs. Of these, Shewanella yellow enzyme 4 (SYE4) is implicated in resistance to oxidative stress. Here, we present a series of high-resolution crystal structures for SYE4 in the oxidized and reduced states, and in complex with phenolic ligands and the nitro-aromatic explosive picric acid. The structures unmask new features, including the identification of a binding platform for long-chain hydrophobic molecules. Furthermore, we present the first structural observation of a hydride-Meisenheimer complex of picric acid with a flavoenzyme. Overall, our study exposes the binding promiscuity of SYE4 toward a variety of electrophilic substrates and is consistent with a general detoxification function for SYE4.

PubMed: 28869767
DOI: 10.1002/1873-3468.12833

実験手法

X-RAY DIFFRACTION (1.55 Å)