5K07

Crystal structure of CREN7-DSDNA (GTAATTGC) complex

5K07 の概要

• エントリーDOI: 10.2210/pdb5k07/pdb
• 関連するPDBエントリー: 5K17
• 分子名称: Chromatin protein Cren7, DNA (5'-D(*GP*TP*AP*AP*TP*TP*GP*C)-3') (3 entities in total)
• 機能のキーワード: protein-dna complex, beta-sheet, dna-binding, methylation, dna binding protein-dna complex, dna binding protein/dna
• 由来する生物種: Sulfolobus solfataricus P2; 詳細
• 細胞内の位置: Cytoplasm : Q97ZE3
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 11561.17

構造登録者

Zhang, Z.F.,Gong, Y. (登録日: 2016-05-17, 公開日: 2017-05-24, 最終更新日: 2023-11-08)

主引用文献

Tian, L.,Zhang, Z.F.,Wang, H.,Zhao, M.,Dong, Y.,Gong, Y.
Sequence-Dependent T:G Base Pair Opening in DNA Double Helix Bound by Cren7, a Chromatin Protein Conserved among Crenarchaea
PLoS ONE, 11:e0163361-e0163361, 2016

PubMed Abstract: T:G base pair arising from spontaneous deamination of 5mC or polymerase errors is a great challenge for DNA repair of hyperthermophilic archaea, especially Crenarchaea. Most strains in this phylum lack the protein homologues responsible for the recognition of the mismatch in the DNA repair pathways. To investigate whether Cren7, a highly conserved chromatin protein in Crenarchaea, serves a role in the repair of T:G mispairs, the crystal structures of Cren7-GTAATTGC and Cren7-GTGATCGC complexes were solved at 2.0 Å and 2.1 Å. In our structures, binding of Cren7 to the AT-rich DNA duplex (GTAATTGC) induces opening of T2:G15 but not T10:G7 base pair. By contrast, both T:G mispairs in the GC-rich DNA duplex (GTGATCGC) retain the classic wobble type. Structural analysis also showed DNA helical changes of GTAATTGC, especially in the steps around the open T:G base pair, as compared to GTGATCGC or the matched DNAs. Surface plasmon resonance assays revealed a 4-fold lower binding affinity of Cren7 for GTAATTGC than that for GTGATCGC, which was dominantly contributed by the decrease of association rate. These results suggested that binding of Cren7 to DNA leads to T:G mispair opening in a sequence dependent manner, and therefore propose the potential roles of Cren7 in DNA repair.

PubMed: 27685992
DOI: 10.1371/journal.pone.0163361

実験手法

X-RAY DIFFRACTION (2 Å)