5JU6

Structural and Functional Studies of Glycoside Hydrolase Family 3 beta-Glucosidase Cel3A from the Moderately Thermophilic Fungus Rasamsonia emersonii

Replaces:  4D0J

Summary for 5JU6

• Entry DOI: 10.2210/pdb5ju6/pdb
• Descriptor: Beta-glucosidase, beta-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (21 entities in total)
• Functional Keywords: beta-glucosidase, glycoprotein, hydrolase
• Biological source: Talaromyces emersonii (Thermophilic fungus)
• Total number of polymer chains: 4
• Total formula weight: 403988.69

Authors

Gudmundsson, M.,Sandgren, M.,Karkehabadi, S. (deposition date: 2016-05-10, release date: 2016-07-13, Last modification date: 2024-01-10)

Primary citation

Gudmundsson, M.,Hansson, H.,Karkehabadi, S.,Larsson, A.,Stals, I.,Kim, S.,Sunux, S.,Fujdala, M.,Larenas, E.,Kaper, T.,Sandgren, M.
Structural and functional studies of the glycoside hydrolase family 3 beta-glucosidase Cel3A from the moderately thermophilic fungus Rasamsonia emersonii.
Acta Crystallogr D Struct Biol, 72:860-870, 2016

PubMed Abstract: The filamentous fungus Hypocrea jecorina produces a number of cellulases and hemicellulases that act in a concerted fashion on biomass and degrade it into monomeric or oligomeric sugars. β-Glucosidases are involved in the last step of the degradation of cellulosic biomass and hydrolyse the β-glycosidic linkage between two adjacent molecules in dimers and oligomers of glucose. In this study, it is shown that substituting the β-glucosidase from H. jecorina (HjCel3A) with the β-glucosidase Cel3A from the thermophilic fungus Rasamsonia emersonii (ReCel3A) in enzyme mixtures results in increased efficiency in the saccharification of lignocellulosic materials. Biochemical characterization of ReCel3A, heterologously produced in H. jecorina, reveals a preference for disaccharide substrates over longer gluco-oligosaccharides. Crystallographic studies of ReCel3A revealed a highly N-glycosylated three-domain dimeric protein, as has been observed previously for glycoside hydrolase family 3 β-glucosidases. The increased thermal stability and saccharification yield and the superior biochemical characteristics of ReCel3A compared with HjCel3A and mixtures containing HjCel3A make ReCel3A an excellent candidate for addition to enzyme mixtures designed to operate at higher temperatures.

PubMed: 27377383
DOI: 10.1107/S2059798316008482

Experimental method

X-RAY DIFFRACTION (2.2 Å)