5JON

Crystal structure of the unliganded form of HCN2 CNBD

5JON の概要

• エントリーDOI: 10.2210/pdb5jon/pdb
• 関連するBIRD辞書のPRD_ID: PRD_900001
• 分子名称: Maltose-binding periplasmic protein,Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2, alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose, NITRATE ION, ... (4 entities in total)
• 機能のキーワード: hcn channels, cyclic nucleotide regulated channels, cyclic nucleotide binding domain, transport protein
• 由来する生物種: Escherichia coli O157:H7; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 115699.19

構造登録者

Klenchin, V.A.,Chanda, B. (登録日: 2016-05-02, 公開日: 2016-11-30, 最終更新日: 2023-09-27)

主引用文献

Goldschen-Ohm, M.P.,Klenchin, V.A.,White, D.S.,Cowgill, J.B.,Cui, Q.,Goldsmith, R.H.,Chanda, B.
Structure and dynamics underlying elementary ligand binding events in human pacemaking channels.
Elife, 5:-, 2016

PubMed Abstract: Although molecular recognition is crucial for cellular signaling, mechanistic studies have relied primarily on ensemble measures that average over and thereby obscure underlying steps. Single-molecule observations that resolve these steps are lacking due to diffraction-limited resolution of single fluorophores at relevant concentrations. Here, we combined zero-mode waveguides with fluorescence resonance energy transfer (FRET) to directly observe binding at individual cyclic nucleotide-binding domains (CNBDs) from human pacemaker ion channels critical for heart and brain function. Our observations resolve the dynamics of multiple distinct steps underlying cyclic nucleotide regulation: a slow initial binding step that must select a 'receptive' conformation followed by a ligand-induced isomerization of the CNBD. X-ray structure of the apo CNBD and atomistic simulations reveal that the isomerization involves both local and global transitions. Our approach reveals fundamental mechanisms underpinning ligand regulation of pacemaker channels, and is generally applicable to weak-binding interactions governing a broad spectrum of signaling processes.

PubMed: 27858593
DOI: 10.7554/eLife.20797

実験手法

X-RAY DIFFRACTION (2.042 Å)