5JO8

CEP104 TOG domain

5JO8 の概要

• エントリーDOI: 10.2210/pdb5jo8/pdb
• 分子名称: CEP104 (2 entities in total)
• 機能のキーワード: centriolar protein, tog domain, cep104, microtubule binder, structural protein
• 由来する生物種: Gallus gallus (Chicken)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 31381.74

構造登録者

Rezabkova, L.,Kraatz, S.H.W. (登録日: 2016-05-02, 公開日: 2016-07-20, 最終更新日: 2024-05-08)

主引用文献

Rezabkova, L.,Kraatz, S.H.,Akhmanova, A.,Steinmetz, M.O.,Kammerer, R.A.
Biophysical and Structural Characterization of the Centriolar Protein Cep104 Interaction Network.
J.Biol.Chem., 291:18496-18504, 2016

PubMed Abstract: Dysfunction of cilia is associated with common genetic disorders termed ciliopathies. Knowledge on the interaction networks of ciliary proteins is therefore key for understanding the processes that are underlying these severe diseases and the mechanisms of ciliogenesis in general. Cep104 has recently been identified as a key player in the regulation of cilia formation. Using a combination of sequence analysis, biophysics, and x-ray crystallography, we obtained new insights into the domain architecture and interaction network of the Cep104 protein. We solved the crystal structure of the tumor overexpressed gene (TOG) domain, identified Cep104 as a novel tubulin-binding protein, and biophysically characterized the interaction of Cep104 with CP110, Cep97, end-binding (EB) protein, and tubulin. Our results represent a solid platform for the further investigation of the microtubule-EB-Cep104-tubulin-CP110-Cep97 network of proteins. Ultimately, such studies should be of importance for understanding the process of cilia formation and the mechanisms underlying different ciliopathies.

PubMed: 27402853
DOI: 10.1074/jbc.M116.739771

実験手法

X-RAY DIFFRACTION (1.4 Å)