5JML

X-ray structure of the complex between bovine pancreatic ribonuclease and penthachlorocarbonyliridate(III) (2 months of soaking)

5JML の概要

• エントリーDOI: 10.2210/pdb5jml/pdb
• 分子名称: Ribonuclease pancreatic, IRIDIUM ION, OXYGEN ATOM, ... (6 entities in total)
• 機能のキーワード: hydrolase, iridium compound, corm, protein iridium interaction
• 由来する生物種: Bos taurus (Bovine)
• 細胞内の位置: Secreted: P61823
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 29259.69

構造登録者

Caterino, M.,Petruk, A.A.,Vergara, A.,Ferraro, G.,Merlino, A. (登録日: 2016-04-29, 公開日: 2016-07-27, 最終更新日: 2024-10-16)

主引用文献

Caterino, M.,Petruk, A.A.,Vergara, A.,Ferraro, G.,Marasco, D.,Doctorovich, F.,Estrin, D.A.,Merlino, A.
Mapping the protein-binding sites for iridium(iii)-based CO-releasing molecules.
Dalton Trans, 45:12206-12214, 2016

PubMed Abstract: A combination of mass spectrometry, Raman microspectroscopy, circular dichroism and X-ray crystallography has been used to obtain detailed information on the reaction of an iridium-based CO-releasing molecule (Ir-CORM), Cs2IrCl5CO, with a model protein, bovine pancreatic ribonuclease. The results show that Ir-compound fragments bind to the N-terminal amine and close to histidine and methionine side chains, and the CO ligand is retained for a long time. The data provide helpful information for identifying protein targets for Ir-CORMs and for studying the mechanism that allows them to exhibit their interesting biological properties.

PubMed: 27411388
DOI: 10.1039/c6dt01685e

実験手法

X-RAY DIFFRACTION (2.29 Å)