5JEU

del-[Ru(phen)2(dppz)]2+ bound to d(TCGGCGCCGA) with Ba2+

5JEU の概要

• エントリーDOI: 10.2210/pdb5jeu/pdb
• 分子名称: DNA (5'-D(*TP*CP*GP*GP*CP*GP*CP*CP*GP*A)-3'), BARIUM ION, Delta-Ru(phen)2(dppz) complex, ... (5 entities in total)
• 機能のキーワード: dna, ruthenium, light-switch, semi-intercalation
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 4706.33

構造登録者

Hall, J.P.,Cardin, C.J. (登録日: 2016-04-19, 公開日: 2016-09-14, 最終更新日: 2024-05-08)

主引用文献

Hall, J.P.,Keane, P.M.,Beer, H.,Buchner, K.,Winter, G.,Sorensen, T.L.,Cardin, D.J.,Brazier, J.A.,Cardin, C.J.
Delta chirality ruthenium 'light-switch' complexes can bind in the minor groove of DNA with five different binding modes.
Nucleic Acids Res., 44:9472-9482, 2016

PubMed Abstract: [Ru(phen)(dppz)] has been studied since the 1990s due to its 'light-switch' properties. It can be used as a luminescent DNA probe, with emission switched on through DNA binding. The luminescence observed is dependent on the solvent accessibility of the pyrazine nitrogen atoms, and therefore is sensitive to changes in both binding site of the cation and chromophore orientation. The compound is also chiral, and there are distinct differences between the enantiomers in terms of the emission behaviour when bound to a variety of DNA sequences. Whilst a number of binary DNA-complex X-ray crystal structures are available, most include the Λ enantiomer and there is very little structural information about binding of the Δ enantiomer. Here, we present the first X-ray crystal structure of a Δ enantiomer bound to well-matched DNA, in the absence of the other, Λ enantiomer. We show how the binding site observed here can be related to a more general pattern of motifs in the crystallographic literature and propose that the Δ enantiomer can bind with five different binding modes, offering a new hypothesis for the interpretation of solution data.

PubMed: 27599841
DOI: 10.1093/nar/gkw753

実験手法

X-RAY DIFFRACTION (0.97 Å)