5JCS

CRYO-EM STRUCTURE OF THE RIX1-REA1 PRE-60S PARTICLE

これはPDB形式変換不可エントリーです。

「5FL8」から置き換えられました

5JCS の概要

• エントリーDOI: 10.2210/pdb5jcs/pdb
• EMDBエントリー: 3199
• 分子名称: 60S ribosomal protein L2-A, 60S ribosomal protein L34-A, 60S ribosomal protein L7-A, ... (48 entities in total)
• 機能のキーワード: pre-60s ribosome rix1 rea1, ribosome
• 由来する生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast); 詳細
• タンパク質・核酸の鎖数: 48
• 化学式量合計: 2814398.17

構造登録者

Barrio-Garcia, C.,Thoms, M.,Flemming, D.,Kater, L.,Berninghausen, O.,Bassler, J.,Beckmann, R.,Hurt, E. (登録日: 2016-04-15, 公開日: 2016-11-16, 最終更新日: 2024-07-10)

主引用文献

Barrio-Garcia, C.,Thoms, M.,Flemming, D.,Kater, L.,Berninghausen, O.,Bassler, J.,Beckmann, R.,Hurt, E.
Architecture of the Rix1-Rea1 checkpoint machinery during pre-60S-ribosome remodeling
Nat.Struct.Mol.Biol., 23:37-44, 2016

PubMed Abstract: Ribosome synthesis is catalyzed by ∼200 assembly factors, which facilitate efficient production of mature ribosomes. Here, we determined the cryo-EM structure of a Saccharomyces cerevisiae nucleoplasmic pre-60S particle containing the dynein-related 550-kDa Rea1 AAA(+) ATPase and the Rix1 subcomplex. This particle differs from its preceding state, the early Arx1 particle, by two massive structural rearrangements: an ∼180° rotation of the 5S ribonucleoprotein complex and the central protuberance (CP) rRNA helices, and the removal of the 'foot' structure from the 3' end of the 5.8S rRNA. Progression from the Arx1 to the Rix1 particle was blocked by mutational perturbation of the Rix1-Rea1 interaction but not by a dominant-lethal Rea1 AAA(+) ATPase-ring mutant. After remodeling, the Rix1 subcomplex and Rea1 become suitably positioned to sense correct structural maturation of the CP, which allows unidirectional progression toward mature ribosomes.

PubMed: 26619264
DOI: 10.1038/nsmb.3132

実験手法

ELECTRON MICROSCOPY (9.5 Å)