5JAW

Structure of a beta galactosidase with inhibitor

5JAW の概要

• エントリーDOI: 10.2210/pdb5jaw/pdb
• 分子名称: Beta-galactosidase, putative, bgl35A, (1S,2S,3S,4S,5R,6R)-5-amino-6-(hydroxymethyl)cyclohexane-1,2,3,4-tetrol, SODIUM ION, ... (5 entities in total)
• 機能のキーワード: beta galactosidase, inhibitor, aziridine, hydrolase
• 由来する生物種: Cellvibrio japonicus (strain Ueda107)
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 499195.56

構造登録者

Offen, W.,Davies, G. (登録日: 2016-04-12, 公開日: 2017-05-10, 最終更新日: 2024-10-23)

主引用文献

Schroder, S.P.,van de Sande, J.W.,Kallemeijn, W.W.,Kuo, C.L.,Artola, M.,van Rooden, E.J.,Jiang, J.,Beenakker, T.J.M.,Florea, B.I.,Offen, W.A.,Davies, G.J.,Minnaard, A.J.,Aerts, J.M.F.G.,Codee, J.D.C.,van der Marel, G.A.,Overkleeft, H.S.
Towards broad spectrum activity-based glycosidase probes: synthesis and evaluation of deoxygenated cyclophellitol aziridines.
Chem. Commun. (Camb.), 53:12528-12531, 2017

PubMed Abstract: Activity-based protein profiling has emerged as a powerful tool for visualizing glycosidases in complex biological samples. Several configurational cyclophellitol isomers have been shown to display high selectivity as probes for glycosidases processing substrates featuring the same configuration. Here, a set of deoxygenated cyclophellitols are presented which enable inter-class profiling of β-glucosidases and β-galactosidases.

PubMed: 29116266
DOI: 10.1039/c7cc07730k

実験手法

X-RAY DIFFRACTION (1.6 Å)