5J93

Five minutes iron loaded Rana Catesbeiana H' ferritin variant E57A/E136A/D140A

5J93 の概要

• エントリーDOI: 10.2210/pdb5j93/pdb
• 関連するPDBエントリー: 5J8S 5J8W
• 分子名称: Ferritin, middle subunit, FE (II) ION, CHLORIDE ION, ... (5 entities in total)
• 機能のキーワード: rana catesbeiana, ferritin variant, ferroxidase activity, m type, h' type, oxidoreductase activity, iron, oxidoreductase
• 由来する生物種: Lithobates catesbeiana (American bullfrog)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 21285.96

構造登録者

Pozzi, C.,Di Pisa, F.,Mangani, S.,Bernacchioni, C.,Turano, P. (登録日: 2016-04-08, 公開日: 2016-10-05, 最終更新日: 2024-01-10)

主引用文献

Bernacchioni, C.,Pozzi, C.,Di Pisa, F.,Mangani, S.,Turano, P.
Ferroxidase Activity in Eukaryotic Ferritin is Controlled by Accessory-Iron-Binding Sites in the Catalytic Cavity.
Chemistry, 22:16213-16219, 2016

PubMed Abstract: Ferritins are iron-storage nanocage proteins that catalyze the oxidation of Fe to Fe at ferroxidase sites. By a combination of structural and spectroscopic techniques, Asp140, together with previously identified Glu57 and Glu136, is demonstrated to be an essential residue to promote the iron oxidation at the ferroxidase site. However, the presence of these three carboxylate moieties in close proximity to the catalytic centers is not essential to achieve binding of the Fe substrate to the diferric ferroxidase sites with the same coordination geometries as in the wild-type cages.

PubMed: 27650996
DOI: 10.1002/chem.201602842

実験手法

X-RAY DIFFRACTION (1.1 Å)