5J8D

Structure of nitroreductase from E. cloacae complexed with nicotinic acid adenine dinucleotide

5J8D の概要

• エントリーDOI: 10.2210/pdb5j8d/pdb
• 関連するPDBエントリー: 1KQB 1KQC 1KQD 5j8g
• 分子名称: Oxygen-insensitive NAD(P)H nitroreductase, FLAVIN MONONUCLEOTIDE, NICOTINIC ACID ADENINE DINUCLEOTIDE, ... (4 entities in total)
• 機能のキーワード: nitroreductase, complex, naad, substrate, oxidoreductase
• 由来する生物種: Enterobacter cloacae
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 99890.97

構造登録者

Haynes, C.A.,Koder, R.L.,Miller, A.F.,Rodgers, D.W. (登録日: 2016-04-07, 公開日: 2017-05-17, 最終更新日: 2023-09-27)

主引用文献

Pitsawong, W.,Haynes, C.A.,Koder, R.L.,Rodgers, D.W.,Miller, A.F.
Mechanism-Informed Refinement Reveals Altered Substrate-Binding Mode for Catalytically Competent Nitroreductase.
Structure, 25:978-987.e4, 2017

PubMed Abstract: Nitroreductase (NR) from Enterobacter cloacae reduces diverse nitroaromatics including herbicides, explosives, and prodrugs, and holds promise for bioremediation, prodrug activation, and enzyme-assisted synthesis. We solved crystal structures of NR complexes with bound substrate or analog for each of its two half-reactions. We complemented these with kinetic isotope effect (KIE) measurements elucidating H-transfer steps essential to each half-reaction. KIEs indicate hydride transfer from NADH to the flavin consistent with our structure of NR with the NADH analog nicotinic acid adenine dinucleotide (NAAD). The KIE on reduction of p-nitrobenzoic acid (p-NBA) also indicates hydride transfer, and requires revision of prior computational mechanisms. Our mechanistic information provided a structural restraint for the orientation of bound substrate, placing the nitro group closer to the flavin N5 in the pocket that binds the amide of NADH. KIEs show that solvent provides a proton, enabling accommodation of different nitro group placements, consistent with the broad repertoire of NR.

PubMed: 28578873
DOI: 10.1016/j.str.2017.05.002

実験手法

X-RAY DIFFRACTION (1.85 Å)