5J7I

Crystal structure of a Geobacillus thermoglucosidasius Acetylating Aldehyde Dehydrogenase in complex with ADP

5J7I の概要

• エントリーDOI: 10.2210/pdb5j7i/pdb
• 関連するPDBエントリー: 5J78
• 分子名称: Acetaldehyde dehydrogenase (Acetylating), ADENOSINE-5'-DIPHOSPHATE, ADENOSINE-5-DIPHOSPHORIBOSE (3 entities in total)
• 機能のキーワード: acetylating aldehyde dehydrogenase, oxidoreductase, complex
• 由来する生物種: Geobacillus thermoglucosidasius (strain C56-YS93)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 210964.61

構造登録者

Crennell, S.J.,Extance, J.P.,Danson, M.J. (登録日: 2016-04-06, 公開日: 2016-09-07, 最終更新日: 2024-11-20)

主引用文献

Extance, J.,Danson, M.J.,Crennell, S.J.
Structure of an acetylating aldehyde dehydrogenase from the thermophilic ethanologen Geobacillus thermoglucosidasius.
Protein Sci., 25:2045-2053, 2016

PubMed Abstract: Acetylating aldehyde dehydrogenases (AcAldDH) catalyse the acetylation of Coenzyme-A (CoA), or in reverse generate acetaldehyde from Acetyl-CoA using NADH as a co-factor. This article reports the expression, purification, enzyme assay, and X-ray crystal structures of an AcAldDH from Geobacillus thermoglucosidasius (GtAcAldDH) to 2.1Å and in complex with CoA and NAD to 4.0Å. In the structure, the AcAldDH forms a close-knit dimer, similar to that seen in other Alcohol Dehydrogenase (ADH) structures. In GtAcAldDH, these dimers associate via their N-termini to form weakly interacting tetramers. This mode of tetrameric association is also seen in an unpublished AcAldDH deposited in the PDB, but is in contrast to all other ADH structures, (including the one other published AcAldDH found in a bacterial microcompartment), in which the dimers bury a large surface area including the C-termini. This novel mode of association sequesters the active sites and potentially reactive acyl-enzyme intermediates in the center of the tetramer. In other respects, the structure is very similar to the other AcAldDH, binding the cofactors in a corresponding fashion. This similarity enabled the identification of a shortened substrate cavity in G. thermoglucosidasius AcAldDH, explaining the limitations on the length of substrate accepted by the enzyme.

PubMed: 27571338
DOI: 10.1002/pro.3027

実験手法

X-RAY DIFFRACTION (4 Å)