5J6F

Crystal structure of DAH7PS-CM complex from Geobacillus sp. with prephenate

5J6F の概要

• エントリーDOI: 10.2210/pdb5j6f/pdb
• 分子名称: 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase, chorismate mutase-isozyme 3, MANGANESE (II) ION, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: tim barrel, dah7ps, dah7p, transferase, lyase, isomerase
• 由来する生物種: Geobacillus sp. GHH01
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 83817.35

構造登録者

Nazmi, A.R.,Othman, M.,Lang, E.J.M.,Bai, Y.,Allison, T.M.,Panjkar, S.,Arcus, V.L.,Parker, E.J. (登録日: 2016-04-04, 公開日: 2016-09-07, 最終更新日: 2023-09-27)

主引用文献

Nazmi, A.R.,Lang, E.J.M.,Bai, Y.,Allison, T.M.,Othman, M.H.,Panjikar, S.,Arcus, V.L.,Parker, E.J.
Interdomain Conformational Changes Provide Allosteric Regulation en Route to Chorismate.
J. Biol. Chem., 291:21836-21847, 2016

PubMed Abstract: Multifunctional proteins play a variety of roles in metabolism. Here, we examine the catalytic function of the combined 3-deoxy-d- heptulosonate-7-phosphate synthase (DAH7PS) and chorismate mutase (CM) from sp. DAH7PS operates at the start of the biosynthetic pathway for aromatic metabolites, whereas CM operates in a dedicated branch of the pathway for the biosynthesis of amino acids tyrosine and phenylalanine. In line with sequence predictions, the two catalytic functions are located in distinct domains, and these two activities can be separated and retain functionality. For the full-length protein, prephenate, the product of the CM reaction, acts as an allosteric inhibitor for the DAH7PS. The crystal structure of the full-length protein with prephenate bound and the accompanying small angle x-ray scattering data reveal the molecular mechanism of the allostery. Prephenate binding results in the tighter association between the dimeric CM domains and the tetrameric DAH7PS, occluding the active site and therefore disrupting DAH7PS function. Acquisition of a physical gating mechanism to control catalytic function through gene fusion appears to be a general mechanism for providing allostery for this enzyme.

PubMed: 27502275
DOI: 10.1074/jbc.M116.741637

実験手法

X-RAY DIFFRACTION (2.75 Å)