5J2O

Crystal structure of the cyan fluorescence protein Cerulean S175G mutant

5J2O の概要

• エントリーDOI: 10.2210/pdb5j2o/pdb
• 分子名称: Green fluorescent protein (2 entities in total)
• 機能のキーワード: cerulean, cyan fluorescent protein, fluorescent protein
• 由来する生物種: Aequorea victoria (Jellyfish)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 27588.09

構造登録者

Park, S.-W.,Kang, S.,Yoon, T.-S. (登録日: 2016-03-29, 公開日: 2016-04-13, 最終更新日: 2026-03-18)

主引用文献

Park, S.-W.,Kang, S.,Yoon, T.-S.
Crystal structure of the cyan fluorescent protein Cerulean-S175G
Acta Crystallogr.,Sect.F, 72:516-522, 2016

PubMed Abstract: Enhanced cyan fluorescent protein (ECFP) was derived from Aequorea victoria green fluorescent protein (avGFP), notably with S65T/Y66W mutations. Its chromophore consists of a tripeptide comprised of Thr65, Trp66 and Gly67 (TWG) residues, while that of avGFP consists of a Ser65, Tyr66 and Gly67 (SYG) tripeptide. Cerulean and SCFP3A were derived from ECFP-S72A/H148D (a double mutation) with additional Y145A and S175G mutations, respectively, while Cerulean-S175G has both mutations (Y145A and S175G). The crystal structures of these ECFP variants at neutral pH were reported to adopt two distinct major conformations called ECFP and Cerulean. In this study, Cerulean-S175G was revealed to adopt only the Cerulean conformation, while Cerulean has been reported to adopt both the ECFP and the Cerulean conformations in its crystal structures. Sharing the same S175G mutation with SCFP3A, Cerulean-S175G showed a slightly increased quantum yield, like SCFP3A, but did not adopt the ECFP conformation adopted by SCFP3A. Detailed comparison of Cerulean-S175G and other ECFP variants revealed that the notable conformational changes in ECFP variants can be understood mainly in terms of the interaction between the Trp66 residue of the chromophore and residues 145-148 of β-strand 7.

PubMed: 27380368
DOI: 10.1107/S2053230X16008311

実験手法

X-RAY DIFFRACTION (1.5 Å)