5J0N

Lambda excision HJ intermediate

5J0N の概要

• エントリーDOI: 10.2210/pdb5j0n/pdb
• EMDBエントリー: 3400
• 分子名称: attP(-117 to +79), attB(-21) to attP(+117), attB(-19 to +21), ... (8 entities in total)
• 機能のキーワード: bacteriophage lambda, excision, site-specific recombination, holliday junction, transferase, hydrolase-dna complex, hydrolase/dna
• 由来する生物種: Enterobacteria phage lambda; 詳細
• タンパク質・核酸の鎖数: 15
• 化学式量合計: 372047.87

構造登録者

Van Duyne, G.,Grigorieff, N.,Landy, A. (登録日: 2016-03-28, 公開日: 2017-02-08, 最終更新日: 2024-03-06)

主引用文献

Laxmikanthan, G.,Xu, C.,Brilot, A.F.,Warren, D.,Steele, L.,Seah, N.,Tong, W.,Grigorieff, N.,Landy, A.,Van Duyne, G.D.
Structure of a Holliday junction complex reveals mechanisms governing a highly regulated DNA transaction.
Elife, 5:-, 2016

PubMed Abstract: The molecular machinery responsible for DNA expression, recombination, and compaction has been difficult to visualize as functionally complete entities due to their combinatorial and structural complexity. We report here the structure of the intact functional assembly responsible for regulating and executing a site-specific DNA recombination reaction. The assembly is a 240-bp Holliday junction (HJ) bound specifically by 11 protein subunits. This higher-order complex is a key intermediate in the tightly regulated pathway for the excision of bacteriophage λ viral DNA out of the E. coli host chromosome, an extensively studied paradigmatic model system for the regulated rearrangement of DNA. Our results provide a structural basis for pre-existing data describing the excisive and integrative recombination pathways, and they help explain their regulation.

PubMed: 27223329
DOI: 10.7554/eLife.14313

実験手法

ELECTRON MICROSCOPY (11 Å)