5J0E

Crystal structures of Pribnow box consensus promoter sequence (P32)

5J0E の概要

• エントリーDOI: 10.2210/pdb5j0e/pdb
• 関連するPDBエントリー: 5F26
• 分子名称: Pribnow box consensus promoter sequence, Complementary sequence, ZINC ION, ... (4 entities in total)
• 機能のキーワード: pribnow box, consensus promoter-sequence, racemic dna, dna crystallography, dna
• 由来する生物種: synthetic construct; 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 22683.18

構造登録者

Mandal, P.K.,Collie, G.W.,Kauffmann, B.,Huc, I. (登録日: 2016-03-28, 公開日: 2016-05-18, 最終更新日: 2024-01-10)

主引用文献

Mandal, P.K.,Collie, G.W.,Srivastava, S.C.,Kauffmann, B.,Huc, I.
Structure elucidation of the Pribnow box consensus promoter sequence by racemic DNA crystallography.
Nucleic Acids Res., 44:5936-5943, 2016

PubMed Abstract: It has previously been shown that the use of racemic mixtures of naturally chiral macromolecules such as protein and DNA can significantly aid the crystallogenesis process, thereby addressing one of the major bottlenecks to structure determination by X-ray crystallographic methods-that of crystal growth. Although previous studies have provided convincing evidence of the applicability of the racemic crystallization technique to DNA through the study of well-characterized DNA structures, we sought to apply this method to a historically challenging DNA sequence. For this purpose we chose a non-self-complementary DNA duplex containing the biologically-relevant Pribnow box consensus sequence 'TATAAT'. Four racemic crystal structures of this previously un-crystallizable DNA target are reported (with resolutions in the range of 1.65-2.3 Å), with further crystallographic studies and structural analysis providing insight into the racemic crystallization process as well as structural details of this highly pertinent DNA sequence.

PubMed: 27137886
DOI: 10.1093/nar/gkw367

実験手法

X-RAY DIFFRACTION (2.81 Å)