5IY4

Crystal structure of human PCNA in complex with the PIP box of DVC1

5IY4 の概要

• エントリーDOI: 10.2210/pdb5iy4/pdb
• 分子名称: Proliferating cell nuclear antigen, DVC1 PIP box (2 entities in total)
• 機能のキーワード: pcna, pip box, dvc1, dna binding protein
• 由来する生物種: Homo sapiens (Human); 詳細
• 細胞内の位置: Nucleus : P12004
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 95568.07

構造登録者

Jiang, T.,Xu, M.,Wang, Y. (登録日: 2016-03-24, 公開日: 2016-06-08, 最終更新日: 2024-10-30)

主引用文献

Wang, Y.,Xu, M.,Jiang, T.
Crystal structure of human PCNA in complex with the PIP box of DVC1
Biochem.Biophys.Res.Commun., 474:264-270, 2016

PubMed Abstract: In higher eukaryotes, DVC1 (SPRTN, Spartan or C1orf124) is implicated in the translesion synthesis (TLS) pathway. DVC1 localizes to sites of DNA damage, binds to the proliferating cell nuclear antigen (PCNA) via its conserved PCNA-interacting motif (PIP box), and associates with ubiquitin selective segregase p97 and other factors, thus regulating translesion synthesis polymerases. Here, we report the crystal structure of human PCNA in complex with a peptide ((321)SNSHQNVLSNYFPRVS(336)) derived from human DVC1 that contains a unique YF type PIP box. Structural analysis reveals the detailed PIP box-PCNA interaction. Interestingly, substitution of Y331 with Phe severely reduces its PCNA binding affinity. These findings offer new insights into the determinants of PIP box for PCNA binding.

PubMed: 27084448
DOI: 10.1016/j.bbrc.2016.04.053

実験手法

X-RAY DIFFRACTION (2.945 Å)