5IQQ

Crystal structure of the human RBM7 RRM domain

5IQQ の概要

• エントリーDOI: 10.2210/pdb5iqq/pdb
• 分子名称: RNA-binding protein 7 (2 entities in total)
• 機能のキーワード: pentameric assembly, rrm fold, non-crystallographic symmetry, rna binding protein, next complex, exosome, rna degradation
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 50583.79

構造登録者

Sofos, N.,Winkler, M.B.L.,Brodersen, D.E. (登録日: 2016-03-11, 公開日: 2016-05-04, 最終更新日: 2024-01-10)

主引用文献

Sofos, N.,Winkler, M.B.,Brodersen, D.E.
RRM domain of human RBM7: purification, crystallization and structure determination.
Acta Crystallogr.,Sect.F, 72:397-402, 2016

PubMed Abstract: RNA decay is an important process that is essential for controlling the abundance, quality and maturation of transcripts. In eukaryotes, RNA decay in the 3'-5' direction is carried out by the exosome, an RNA-degradation machine that is conserved from yeast to humans. A range of cofactors stimulate the enzymatic activity of the exosome and serve as adapters for the many RNA substrates. In human cells, the exosome associates with the heterotrimeric nuclear exosome targeting (NEXT) complex consisting of the DExH-box helicase hMTR4, the zinc-finger protein hZCCHC8 and the RRM-type protein hRBM7. Here, the 2.5 Å resolution crystal structure of the RRM domain of human RBM7 is reported. Molecular replacement using a previously determined solution structure of RBM7 was unsuccessful. Instead, RBM8 and CBP20 RRM-domain crystal structures were used to successfully determine the RBM7 structure by molecular replacement. The structure reveals a ring-shaped pentameric assembly, which is most likely a consequence of crystal packing.

PubMed: 27139832
DOI: 10.1107/S2053230X16006129

実験手法

X-RAY DIFFRACTION (2.52 Å)