5IPX

Structure of ORF49 from KSHV

5IPX の概要

• エントリーDOI: 10.2210/pdb5ipx/pdb
• 分子名称: ORF49 protein, SULFATE ION (3 entities in total)
• 機能のキーワード: orf49, kshv, herpesvirus, lytic replication, viral protein
• 由来する生物種: Human herpesvirus 8 (HHV-8)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 37489.75

構造登録者

Hew, K.,Nordlund, P. (登録日: 2016-03-10, 公開日: 2016-11-16, 最終更新日: 2024-03-20)

主引用文献

Hew, K.,Veerappan, S.,Sim, D.,Cornvik, T.,Nordlund, P.,Dahlroth, S.L.
Structure of the Open Reading Frame 49 Protein Encoded by Kaposi's Sarcoma-Associated Herpesvirus.
J. Virol., 91:-, 2017

PubMed Abstract: Herpesviruses alternate between the latent and the lytic life cycle. Switching into the lytic life cycle is important for herpesviral replication and disease pathogenesis. Activation of a transcription factor replication and transcription activator (RTA) has been demonstrated to govern this switch in Kaposi's sarcoma-associated herpesvirus (KSHV). The protein encoded by open reading frame 49 from KSHV (ORF49) has been shown to upregulate lytic replication in KSHV by enhancing the activities of the RTA. We have solved the crystal structure of the ORF49 protein to a resolution of 2.4 Å. The ORF49 protein has a novel fold consisting of 12 alpha-helices bundled into two pseudodomains. Most notably are distinct charged patches on the protein surface, which are possible protein-protein interaction sites. Homologs of the ORF49 protein in the gammaherpesvirus subfamily have low sequence similarities. Conserved residues are mainly located in the hydrophobic regions, suggesting that they are more likely to play important structural roles than functional ones. Based on the identification and position of three sulfates binding to the positive areas, we performed some initial protein-DNA binding studies by analyzing the thermal stabilization of the protein in the presence of DNA. The ORF49 protein is stabilized in a dose-responsive manner by double-stranded oligonucleotides, suggesting actual DNA interaction and binding. Biolayer interferometry studies also demonstrated that the ORF49 protein binds these oligonucleotides.

PubMed: 27807232
DOI: 10.1128/JVI.01947-16

実験手法

X-RAY DIFFRACTION (2.4 Å)