5IM5

Crystal structure of designed two-component self-assembling icosahedral cage I53-40

Summary for 5IM5

DescriptorDesigned Riboflavin synthase, Designed Keto-hydroxyglutarate-aldolase/keto-deoxy-phosphogluconate aldolase (2 entities in total)
Functional Keywordsicosahedron, designed protein cage, two-component, computational design, protein engineering, rosetta, self-assembling, co-assembling, multimerization, symmetry, nanomaterial, nanostructure, protein binding
Biological sourceMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)
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Total number of polymer chains30
Total molecular weight606001.57
Authors
Liu, Y.A.,Cascio, D.,Sawaya, M.R.,Bale, J.B.,Collazo, M.J.,Thomas, C.,Sheffler, W.,King, N.P.,Baker, D.,Yeates, T.O. (deposition date: 2016-03-05, release date: 2016-07-27, Last modification date: 2016-08-10)
Primary citation
Bale, J.B.,Gonen, S.,Liu, Y.,Sheffler, W.,Ellis, D.,Thomas, C.,Cascio, D.,Yeates, T.O.,Gonen, T.,King, N.P.,Baker, D.
Accurate design of megadalton-scale two-component icosahedral protein complexes.
Science, 353:389-394, 2016
PubMed: 27463675 (PDB entries with the same primary citation)
DOI: 10.1126/science.aaf8818
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (3.699 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.2481202.7%0.3%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution
Download full validation report