Loading
PDBj
メニューPDBj@FacebookPDBj@X(formerly Twitter)PDBj@BlueSkyPDBj@YouTubewwPDB FoundationwwPDBDonate
RCSB PDBPDBeBMRBAdv. SearchSearch help

5IM5

Crystal structure of designed two-component self-assembling icosahedral cage I53-40

5IM5 の概要
エントリーDOI10.2210/pdb5im5/pdb
分子名称Designed Riboflavin synthase, Designed Keto-hydroxyglutarate-aldolase/keto-deoxy-phosphogluconate aldolase (2 entities in total)
機能のキーワードicosahedron, designed protein cage, two-component, computational design, protein engineering, rosetta, self-assembling, co-assembling, multimerization, symmetry, nanomaterial, nanostructure, protein binding
由来する生物種Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)
詳細
タンパク質・核酸の鎖数30
化学式量合計606001.57
構造登録者
Liu, Y.A.,Cascio, D.,Sawaya, M.R.,Bale, J.B.,Collazo, M.J.,Thomas, C.,Sheffler, W.,King, N.P.,Baker, D.,Yeates, T.O. (登録日: 2016-03-05, 公開日: 2016-07-27, 最終更新日: 2023-09-27)
主引用文献Bale, J.B.,Gonen, S.,Liu, Y.,Sheffler, W.,Ellis, D.,Thomas, C.,Cascio, D.,Yeates, T.O.,Gonen, T.,King, N.P.,Baker, D.
Accurate design of megadalton-scale two-component icosahedral protein complexes.
Science, 353:389-394, 2016
Cited by
PubMed Abstract: Nature provides many examples of self- and co-assembling protein-based molecular machines, including icosahedral protein cages that serve as scaffolds, enzymes, and compartments for essential biochemical reactions and icosahedral virus capsids, which encapsidate and protect viral genomes and mediate entry into host cells. Inspired by these natural materials, we report the computational design and experimental characterization of co-assembling, two-component, 120-subunit icosahedral protein nanostructures with molecular weights (1.8 to 2.8 megadaltons) and dimensions (24 to 40 nanometers in diameter) comparable to those of small viral capsids. Electron microscopy, small-angle x-ray scattering, and x-ray crystallography show that 10 designs spanning three distinct icosahedral architectures form materials closely matching the design models. In vitro assembly of icosahedral complexes from independently purified components occurs rapidly, at rates comparable to those of viral capsids, and enables controlled packaging of molecular cargo through charge complementarity. The ability to design megadalton-scale materials with atomic-level accuracy and controllable assembly opens the door to a new generation of genetically programmable protein-based molecular machines.
PubMed: 27463675
DOI: 10.1126/science.aaf8818
主引用文献が同じPDBエントリー
実験手法
X-RAY DIFFRACTION (3.699 Å)
構造検証レポート
Validation report summary of 5im5
検証レポート(詳細版)ダウンロードをダウンロード

239149

件を2025-07-23に公開中

PDB statisticsPDBj update infoContact PDBjnumon