5IJN

Composite structure of the inner ring of the human nuclear pore complex (32 copies of Nup205)

5IJN の概要

• エントリーDOI: 10.2210/pdb5ijn/pdb
• EMDBエントリー: 8085
• 分子名称: NUCLEAR PORE COMPLEX PROTEIN NUP155, NUCLEAR PORE COMPLEX PROTEIN NUP93, NUCLEAR PORE COMPLEX PROTEIN NUP205, ... (6 entities in total)
• 機能のキーワード: nuclear pore complex, nucleocytoplasmic transport, transport protein
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 26
• 化学式量合計: 2898120.43

構造登録者

Kosinski, J.,Mosalaganti, S.,von Appen, A.,Beck, M. (登録日: 2016-03-02, 公開日: 2016-04-27, 最終更新日: 2024-05-08)

主引用文献

Kosinski, J.,Mosalaganti, S.,von Appen, A.,Teimer, R.,DiGuilio, A.L.,Wan, W.,Bui, K.H.,Hagen, W.J.,Briggs, J.A.,Glavy, J.S.,Hurt, E.,Beck, M.
Molecular architecture of the inner ring scaffold of the human nuclear pore complex.
Science, 352:363-365, 2016

PubMed Abstract: Nuclear pore complexes (NPCs) are 110-megadalton assemblies that mediate nucleocytoplasmic transport. NPCs are built from multiple copies of ~30 different nucleoporins, and understanding how these nucleoporins assemble into the NPC scaffold imposes a formidable challenge. Recently, it has been shown how the Y complex, a prominent NPC module, forms the outer rings of the nuclear pore. However, the organization of the inner ring has remained unknown until now. We used molecular modeling combined with cross-linking mass spectrometry and cryo-electron tomography to obtain a composite structure of the inner ring. This architectural map explains the vast majority of the electron density of the scaffold. We conclude that despite obvious differences in morphology and composition, the higher-order structure of the inner and outer rings is unexpectedly similar.

PubMed: 27081072
DOI: 10.1126/science.aaf0643

実験手法

ELECTRON MICROSCOPY (21.4 Å)