5IJA

[NiFe] hydrogenase maturation protease HybD from Thermococcus kodakarensis

5IJA の概要

• エントリーDOI: 10.2210/pdb5ija/pdb
• 分子名称: Hydrogenase-specific maturation endopeptidase (2 entities in total)
• 機能のキーワード: hydrogenase maturation protease, hydrolase
• 由来する生物種: Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 32668.23

構造登録者

Kwon, S.,Nishitani, Y.,Watanabe, S.,Miki, K. (登録日: 2016-03-01, 公開日: 2016-06-01, 最終更新日: 2023-11-08)

主引用文献

Kwon, S.,Nishitani, Y.,Watanabe, S.,Hirao, Y.,Imanaka, T.,Kanai, T.,Atomi, H.,Miki, K.
Crystal structure of a [NiFe] hydrogenase maturation protease HybD from Thermococcus kodakarensis KOD1
Proteins, 84:1321-1327, 2016

PubMed Abstract: A [NiFe] hydrogenase maturation protease HybD from Thermococcus kodakarensis KOD1 (TkHybD) is involved in the cleavage of the C-terminal residues of [NiFe] hydrogenase large subunits by Ni recognition. Here, we report the crystal structure of TkHybD at 1.82 Å resolution to better understand this process. TkHybD exhibits an α/β/α sandwich fold with conserved residues responsible for the Ni recognition. Comparisons of TkHybD with homologous proteins also reveal that they share a common overall architecture, suggesting that they have similar catalytic functions. Our results including metal binding site prediction provide insight into the substrate recognition and catalysis mechanism of TkHybD. Proteins 2016; 84:1321-1327. © 2016 Wiley Periodicals, Inc.

PubMed: 27192667
DOI: 10.1002/prot.25070

実験手法

X-RAY DIFFRACTION (1.82 Å)