5IHS

Structure of CHU_2103 from Cytophaga hutchinsonii

5IHS の概要

• エントリーDOI: 10.2210/pdb5ihs/pdb
• 分子名称: Endoglucanase, glycoside hydrolase family 5 protein (2 entities in total)
• 機能のキーワード: endoglucanase, tim barrel, hydrolase
• 由来する生物種: Cytophaga hutchinsonii (strain ATCC 33406 / NCIMB 9469)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 38933.45

構造登録者

Silvaggi, N.R.,Han, L. (登録日: 2016-02-29, 公開日: 2016-06-22, 最終更新日: 2023-09-27)

主引用文献

Zhu, Y.,Han, L.,Hefferon, K.L.,Silvaggi, N.R.,Wilson, D.B.,McBride, M.J.
Periplasmic Cytophaga hutchinsonii Endoglucanases Are Required for Use of Crystalline Cellulose as the Sole Source of Carbon and Energy.
Appl.Environ.Microbiol., 82:4835-4845, 2016

PubMed Abstract: The soil bacterium Cytophaga hutchinsonii actively digests crystalline cellulose by a poorly understood mechanism. Genome analyses identified nine genes predicted to encode endoglucanases with roles in this process. No predicted cellobiohydrolases, which are usually involved in the utilization of crystalline cellulose, were identified. Chromosomal deletions were performed in eight of the endoglucanase-encoding genes: cel5A, cel5B, cel5C, cel9A, cel9B, cel9C, cel9E, and cel9F Each mutant retained the ability to digest crystalline cellulose, although the deletion of cel9C caused a modest decrease in cellulose utilization. Strains with multiple deletions were constructed to identify the critical cellulases. Cells of a mutant lacking both cel5B and cel9C were completely deficient in growth on cellulose. Cell fractionation and biochemical analyses indicate that Cel5B and Cel9C are periplasmic nonprocessive endoglucanases. The requirement of periplasmic endoglucanases for cellulose utilization suggests that cellodextrins are transported across the outer membrane during this process. Bioinformatic analyses predict that Cel5A, Cel9A, Cel9B, Cel9D, and Cel9E are secreted across the outer membrane by the type IX secretion system, which has been linked to cellulose utilization. These secreted endoglucanases may perform the initial digestion within amorphous regions on the cellulose fibers, releasing oligomers that are transported into the periplasm for further digestion by Cel5B and Cel9C. The results suggest that both cell surface and periplasmic endoglucanases are required for the growth of C. hutchinsonii on cellulose and that novel cell surface proteins may solubilize and transport cellodextrins across the outer membrane.

PubMed: 27260354
DOI: 10.1128/AEM.01298-16

実験手法

X-RAY DIFFRACTION (1.1 Å)