5IE8

The pyrazinoic acid binding domain of Ribosomal Protein S1 from Mycobacterium tuberculosis

5IE8 の概要

• エントリーDOI: 10.2210/pdb5ie8/pdb
• NMR情報: BMRB: 26725
• 分子名称: 30S ribosomal protein S1 (1 entity in total)
• 機能のキーワード: tuberculosis, mtrpsa, trans-translation, poa, translation
• 由来する生物種: Mycobacterium leprae (strain TN)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 9992.36

構造登録者

Huang, B.,Liao, X. (登録日: 2016-02-25, 公開日: 2016-03-16, 最終更新日: 2024-05-15)

主引用文献

Huang, B.,Fu, J.,Guo, C.,Wu, X.,Lin, D.,Liao, X.
(1)H, (15)N, (13)C resonance assignments for pyrazinoic acid binding domain of ribosomal protein S1 from Mycobacterium tuberculosis
Biomol NMR Assign, 10:321-324, 2016

PubMed Abstract: Ribosomal protein S1 of Mycobacterium tuberculosis (MtRpsA) binds to ribosome and mRNA, and plays significant role in the regulation of translation initiation, conventional protein synthesis and transfer-messenger RNA (tmRNA) mediated trans-translation. It has been identified as the target of pyrazinoic acid (POA), a bactericidal moiety from hydrolysis of pyrazinamide, which is a mainstay of combination therapy for tuberculosis. POA prevented the interactions between the C-terminal S1 domain of MtRpsA (residues 280-368, MtRpsA(CTD)_S1) and tmRNA; so that POA can inhibit the trans-translation, which is a key component of multiple quality control pathways in bacteria. However, the details of molecular mechanism and dynamic characteristics for MtRpsA(CTD)_S1 interactions with POA, tmRNA or mRNA are still unclear. Here we present the (1)H, (15)N, (13)C resonance assignments of MtRpsA(CTD)_S1 as well as the secondary structure information based on backbone chemical shifts, which lay foundation for further solution structure determination, dynamic properties characterization and interactions investigation between MtRpsA(CTD)_S1 and tmRNA, RNA or POA.

PubMed: 27412769
DOI: 10.1007/s12104-016-9692-9

実験手法

SOLUTION NMR