5ID3

Solution structure of the pore-forming region of C. elegans Mitochondrial Calcium Uniporter (MCU)

Summary for 5ID3

• Entry DOI: 10.2210/pdb5id3/pdb
• NMR Information: BMRB: 30021
• Descriptor: Mitochondrial Calcium Uniporter (1 entity in total)
• Functional Keywords: calcium channel, mitochondria, pentamer, n-terminal domain truncation, structural genomics, psi-biology, membrane protein structures by solution nmr, mpsbynmr, transport protein
• Biological source: Caenorhabditis elegans
• Total number of polymer chains: 5
• Total formula weight: 94636.03

Authors

Oxenoid, K.,Dong, Y.,Cao, C.,Cui, T.,Sancak, Y.,Markhard, A.L.,Grabarek, Z.,Kong, L.,Liu, Z.,Ouyang, B.,Cong, Y.,Mootha, V.K.,Chou, J.J.,Membrane Protein Structures by Solution NMR (MPSbyNMR) (deposition date: 2016-02-23, release date: 2016-05-04, Last modification date: 2024-05-15)

Primary citation

Oxenoid, K.,Dong, Y.,Cao, C.,Cui, T.,Sancak, Y.,Markhard, A.L.,Grabarek, Z.,Kong, L.,Liu, Z.,Ouyang, B.,Cong, Y.,Mootha, V.K.,Chou, J.J.
Architecture of the mitochondrial calcium uniporter.
Nature, 533:269-273, 2016

PubMed Abstract: Mitochondria from many eukaryotic clades take up large amounts of calcium (Ca(2+)) via an inner membrane transporter called the uniporter. Transport by the uniporter is membrane potential dependent and sensitive to ruthenium red or its derivative Ru360 (ref. 1). Electrophysiological studies have shown that the uniporter is an ion channel with remarkably high conductance and selectivity. Ca(2+) entry into mitochondria is also known to activate the tricarboxylic acid cycle and seems to be crucial for matching the production of ATP in mitochondria with its cytosolic demand. Mitochondrial calcium uniporter (MCU) is the pore-forming and Ca(2+)-conducting subunit of the uniporter holocomplex, but its primary sequence does not resemble any calcium channel studied to date. Here we report the structure of the pore domain of MCU from Caenorhabditis elegans, determined using nuclear magnetic resonance (NMR) and electron microscopy (EM). MCU is a homo-oligomer in which the second transmembrane helix forms a hydrophilic pore across the membrane. The channel assembly represents a new solution of ion channel architecture, and is stabilized by a coiled-coil motif protruding into the mitochondrial matrix. The critical DXXE motif forms the pore entrance, which features two carboxylate rings; based on the ring dimensions and functional mutagenesis, these rings appear to form the selectivity filter. To our knowledge, this is one of the largest membrane protein structures characterized by NMR, and provides a structural blueprint for understanding the function of this channel.

PubMed: 27135929
DOI: 10.1038/nature17656

Experimental method

SOLUTION NMR