5I32

Ammonia permeable aquaporin AtTIP2;1

5I32 の概要

• エントリーDOI: 10.2210/pdb5i32/pdb
• 分子名称: Aquaporin TIP2-1 (2 entities in total)
• 機能のキーワード: aquaporin, mip, ammonia-permeable, membrane protein
• 由来する生物種: Arabidopsis thaliana (Mouse-ear cress)
• 細胞内の位置: Vacuole membrane ; Multi-pass membrane protein : Q41951
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 28043.02

構造登録者

Kirscht, A.,Nissen, P.,Kjellbom, P.,Gourdon, P.,Johanson, U. (登録日: 2016-02-09, 公開日: 2016-04-06, 最終更新日: 2024-01-10)

主引用文献

Kirscht, A.,Kaptan, S.S.,Bienert, G.P.,Chaumont, F.,Nissen, P.,de Groot, B.L.,Kjellbom, P.,Gourdon, P.,Johanson, U.
Crystal Structure of an Ammonia-Permeable Aquaporin.
Plos Biol., 14:e1002411-e1002411, 2016

PubMed Abstract: Aquaporins of the TIP subfamily (Tonoplast Intrinsic Proteins) have been suggested to facilitate permeation of water and ammonia across the vacuolar membrane of plants, allowing the vacuole to efficiently sequester ammonium ions and counteract cytosolic fluctuations of ammonia. Here, we report the structure determined at 1.18 Å resolution from twinned crystals of Arabidopsis thaliana aquaporin AtTIP2;1 and confirm water and ammonia permeability of the purified protein reconstituted in proteoliposomes as further substantiated by molecular dynamics simulations. The structure of AtTIP2;1 reveals an extended selectivity filter with the conserved arginine of the filter adopting a unique unpredicted position. The relatively wide pore and the polar nature of the selectivity filter clarify the ammonia permeability. By mutational studies, we show that the identified determinants in the extended selectivity filter region are sufficient to convert a strictly water-specific human aquaporin into an AtTIP2;1-like ammonia channel. A flexible histidine and a novel water-filled side pore are speculated to deprotonate ammonium ions, thereby possibly increasing permeation of ammonia. The molecular understanding of how aquaporins facilitate ammonia flux across membranes could potentially be used to modulate ammonia losses over the plasma membrane to the atmosphere, e.g., during photorespiration, and thereby to modify the nitrogen use efficiency of plants.

PubMed: 27028365
DOI: 10.1371/journal.pbio.1002411

実験手法

X-RAY DIFFRACTION (1.18 Å)