5I0W

IRON AND COPPER-BOUND P19 FROM CAMPYLOBACTER JEJUNI UNDER REDUCING CONDITIONS

Summary for 5I0W

• Entry DOI: 10.2210/pdb5i0w/pdb
• Related: 3LZN 3LZO 3LZQ 5I0V 5I0X 5I0Y
• Descriptor: P19, TRIS(HYDROXYETHYL)AMINOMETHANE, COPPER (I) ION, ... (6 entities in total)
• Functional Keywords: mixed beta sandwich, iron transport, metal transport
• Biological source: Campylobacter jejuni subsp. jejuni 81-176
• Total number of polymer chains: 2
• Total formula weight: 35598.78

Authors

Chan, A.C.,Murphy, M.E. (deposition date: 2016-02-04, release date: 2017-02-08, Last modification date: 2023-09-27)

Primary citation

Chan, A.C.K.,Lin, H.,Koch, D.,Grass, G.,Nies, D.H.,Murphy, M.E.P.
A copper site is required for iron transport by the periplasmic proteins P19 and FetP.
Metallomics, 12:1530-1541, 2020

PubMed Abstract: Campylobacter jejuni is a leading cause of food-borne gastrointestinal disease in humans and uropathogenic Escherichia coli is a leading cause of urinary tract infections. Both human pathogens harbour a homologous iron uptake system (termed cjFetM-P19 in C. jejuni and ecFetM-FetP in E. coli). Although these systems are important for growth under iron limitation, the mechanisms by which these systems function during iron transport remain undefined. The copper ions bound to P19 and FetP, the homologous periplasmic proteins, are coordinated in an uncommon penta-dentate manner involving a Met-Glu-His3 motif and exhibit positional plasticity. Here we demonstrate the function of the Met and Glu residues in modulating copper binding and controlling copper positioning through site-directed variants, binding assays, and crystal structures. Growth of C. jejuni strains with these p19 variants is impaired under iron limited conditions as compared to the wild-type strain. Additionally, an acidic residue-rich secondary site is required for binding iron and function in vivo. Finally, western blot analyses demonstrate direct and specific interactions between periplasmic P19 and FetP with the large periplasmic domain of their respective inner membrane transporters cjFetM and ecFetM.

PubMed: 32780051
DOI: 10.1039/d0mt00130a

Experimental method

X-RAY DIFFRACTION (1.55 Å)