5HZT

Crystal structure of Dronpa-Cu2+

5HZT の概要

• エントリーDOI: 10.2210/pdb5hzt/pdb
• 関連するPDBエントリー: 2GX2 5HZS 5HZU
• 分子名称: Fluorescent protein Dronpa, COPPER (II) ION (3 entities in total)
• 機能のキーワード: fluorescent protein, luminescent protein
• 由来する生物種: Echinophyllia sp. SC22
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 297382.52

構造登録者

Hwang, K.Y.,Nam, K.H. (登録日: 2016-02-03, 公開日: 2017-03-15, 最終更新日: 2026-03-18)

主引用文献

Kim, I.J.,Kim, S.,Park, J.,Eom, I.,Kim, S.,Kim, J.H.,Ha, S.C.,Kim, Y.G.,Hwang, K.Y.,Nam, K.H.
Crystal structures of Dronpa complexed with quenchable metal ions provide insight into metal biosensor development
FEBS Lett., 590:2982-2990, 2016

PubMed Abstract: Many fluorescent proteins (FPs) show fluorescence quenching by specific metal ions, which can be applied towards metal biosensor development. In this study, we investigated the significant fluorescence quenching of Dronpa by Co(2+) and Cu(2+) ions. Crystal structures of Co(2+) -, Ni(2+) - and Cu(2+) -bound Dronpa revealed previously unseen, unique, metal-binding sites for fluorescence quenching. These metal ions commonly interact with surface-exposed histidine residues (His194-His210 and His210-His212), and interact indirectly with chromophores. Structural analysis of the Co(2+) - and Cu(2+) - binding sites of Dronpa provides insight into FP-based metal biosensor engineering.

PubMed: 27433793
DOI: 10.1002/1873-3468.12316

実験手法

X-RAY DIFFRACTION (2.84 Å)