5HYM

3-Hydroxybenzoate 6-hydroxylase from Rhodococcus jostii in complex with phosphatidylinositol

5HYM の概要

• エントリーDOI: 10.2210/pdb5hym/pdb
• 分子名称: Probable salicylate monooxygenase, FLAVIN-ADENINE DINUCLEOTIDE, Phosphatidylinositol, ... (5 entities in total)
• 機能のキーワード: flavoprotein, monooxygenase, phospholipid, rhodococcus, oxidoreductase
• 由来する生物種: Rhodococcus jostii (strain RHA1)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 48656.37

構造登録者

Orru, R.,Montersino, S.,Mattevi, A.,van Berkel, W.J.H. (登録日: 2016-02-01, 公開日: 2017-02-01, 最終更新日: 2024-01-10)

主引用文献

Montersino, S.,Te Poele, E.,Orru, R.,Westphal, A.H.,Barendregt, A.,Heck, A.J.R.,van der Geize, R.,Dijkhuizen, L.,Mattevi, A.,van Berkel, W.J.H.
3-Hydroxybenzoate 6-Hydroxylase from Rhodococcus jostii RHA1 Contains a Phosphatidylinositol Cofactor.
Front Microbiol, 8:1110-1110, 2017

PubMed Abstract: 3-Hydroxybenzoate 6-hydroxylase (3HB6H, EC 1.13.14.26) is a FAD-dependent monooxygenase involved in the catabolism of aromatic compounds in soil microorganisms. 3HB6H is unique among flavoprotein hydroxylases in that it harbors a phospholipid ligand. The purified protein obtained from expressing the gene encoding 3HB6H from RHA1 in the host contains a mixture of phosphatidylglycerol and phosphatidylethanolamine, which are the major constituents of cytoplasmic membrane. Here, we purified 3HB6H (HB6H) produced in the host RHA#2 by employing a newly developed actinomycete expression system. Biochemical and biophysical analysis revealed that 3HB6H possesses similar catalytic and structural features as 3HB6H, but now contains phosphatidylinositol, which is a specific constituent of actinomycete membranes. Native mass spectrometry suggests that the lipid cofactor stabilizes monomer-monomer contact. Lipid analysis of 3HB6H from NCIMB 9867 (3HB6H) produced in supports the conclusion that 3HB6H enzymes have an intrinsic ability to bind phospholipids with different specificity, reflecting the membrane composition of their bacterial host.

PubMed: 28670303
DOI: 10.3389/fmicb.2017.01110

実験手法

X-RAY DIFFRACTION (2.3 Å)