5HXY

Crystal structure of XerA recombinase

5HXY の概要

• エントリーDOI: 10.2210/pdb5hxy/pdb
• 分子名称: Tyrosine recombinase XerA, PHOSPHATE ION (3 entities in total)
• 機能のキーワード: recombinase, xera, recombination
• 由来する生物種: Thermoplasma acidophilum DSM 1728
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 223703.03

構造登録者

Hwang, K.Y.,Nam, K.H. (登録日: 2016-01-31, 公開日: 2017-02-01, 最終更新日: 2024-11-20)

主引用文献

Jo, C.H.,Kim, J.,Han, A.R.,Park, S.Y.,Hwang, K.Y.,Nam, K.H.
Crystal structure of Thermoplasma acidophilum XerA recombinase shows large C-shape clamp conformation and cis-cleavage mode for nucleophilic tyrosine
FEBS Lett., 590:848-856, 2016

PubMed Abstract: Site-specific Xer recombination plays a pivotal role in reshuffling genetic information. Here, we report the 2.5 Å crystal structure of XerA from the archaean Thermoplasma acidophilum. Crystallographic data reveal a uniquely open conformational state, resulting in a C-shaped clamp with an angle of ~ 48° and a distance of 57 Å between the core-binding and the catalytic domains. The catalytic nucleophile, Tyr264, is positioned in cis-cleavage mode by XerA's C-term tail that interacts with the CAT domain of a neighboring monomer without DNA substrate. Structural comparisons of tyrosine recombinases elucidate the dynamics of Xer recombinase.

PubMed: 26919387
DOI: 10.1002/1873-3468.12109

実験手法

X-RAY DIFFRACTION (2.5 Å)