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5HV9

Human LTC4S mutant-S36E

Summary for 5HV9
Entry DOI10.2210/pdb5hv9/pdb
DescriptorLeukotriene C4 synthase, GLUTATHIONE, SULFATE ION (3 entities in total)
Functional Keywordsltc4s, mutant, lyase
Biological sourceHomo sapiens (Human)
Cellular locationNucleus outer membrane; Multi-pass membrane protein: Q16873
Total number of polymer chains1
Total formula weight17856.97
Authors
Thulasingam, M.,Ahmad, H.R.S.,Rinaldo-Matthis, A.,Haeggstrom, J.Z. (deposition date: 2016-01-28, release date: 2016-07-13, Last modification date: 2024-01-10)
Primary citationAhmad, S.,Ytterberg, A.J.,Thulasingam, M.,Tholander, F.,Bergman, T.,Zubarev, R.,Wetterholm, A.,Rinaldo-Matthis, A.,Haeggstrom, J.Z.
Phosphorylation of Leukotriene C4 Synthase at Serine 36 Impairs Catalytic Activity.
J.Biol.Chem., 291:18410-18418, 2016
Cited by
PubMed Abstract: Leukotriene C4 synthase (LTC4S) catalyzes the formation of the proinflammatory lipid mediator leukotriene C4 (LTC4). LTC4 is the parent molecule of the cysteinyl leukotrienes, which are recognized for their pathogenic role in asthma and allergic diseases. Cellular LTC4S activity is suppressed by PKC-mediated phosphorylation, and recently a downstream p70S6k was shown to play an important role in this process. Here, we identified Ser(36) as the major p70S6k phosphorylation site, along with a low frequency site at Thr(40), using an in vitro phosphorylation assay combined with mass spectrometry. The functional consequences of p70S6k phosphorylation were tested with the phosphomimetic mutant S36E, which displayed only about 20% (20 μmol/min/mg) of the activity of WT enzyme (95 μmol/min/mg), whereas the enzyme activity of T40E was not significantly affected. The enzyme activity of S36E increased linearly with increasing LTA4 concentrations during the steady-state kinetics analysis, indicating poor lipid substrate binding. The Ser(36) is located in a loop region close to the entrance of the proposed substrate binding pocket. Comparative molecular dynamics indicated that Ser(36) upon phosphorylation will pull the first luminal loop of LTC4S toward the neighboring subunit of the functional homotrimer, thereby forming hydrogen bonds with Arg(104) in the adjacent subunit. Because Arg(104) is a key catalytic residue responsible for stabilization of the glutathione thiolate anion, this phosphorylation-induced interaction leads to a reduction of the catalytic activity. In addition, the positional shift of the loop and its interaction with the neighboring subunit affect active site access. Thus, our mutational and kinetic data, together with molecular simulations, suggest that phosphorylation of Ser(36) inhibits the catalytic function of LTC4S by interference with the catalytic machinery.
PubMed: 27365393
DOI: 10.1074/jbc.M116.735647
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (3 Å)
Structure validation

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数据于2025-07-02公开中

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