5HUJ

Crystal Structure of NadE from Streptococcus pyogenes

Summary for 5HUJ

• Entry DOI: 10.2210/pdb5huj/pdb
• Related: 5HUH 5HUL 5HUO 5HUP
• Descriptor: NH(3)-dependent NAD(+) synthetase, CHLORIDE ION (3 entities in total)
• Functional Keywords: transferase
• Biological source: Streptococcus pyogenes serotype M49 (strain NZ131)
• Total number of polymer chains: 2
• Total formula weight: 67790.35

Authors

Booth, W.T.,Chruszcz, M. (deposition date: 2016-01-27, release date: 2017-01-25, Last modification date: 2023-09-27)

Primary citation

Booth, W.T.,Morris, T.L.,Mysona, D.P.,Shah, M.J.,Taylor, L.K.,Karlin, T.W.,Clary, K.,Majorek, K.A.,Offermann, L.R.,Chruszcz, M.
Streptococcus pyogenes quinolinate-salvage pathway-structural and functional studies of quinolinate phosphoribosyl transferase and NH3 -dependent NAD(+) synthetase.
FEBS J., 284:2425-2441, 2017

PubMed Abstract: Streptococcus pyogenes, also known as Group A Strep (GAS), is an obligate human pathogen that is responsible for millions of infections and numerous deaths per year. Infection manifestations can range from simple, acute pharyngitis to more complex, necrotizing fasciitis. To date, most treatments for GAS infections involve the use of common antibiotics including tetracycline and clindamycin. Unfortunately, new strains have been identified that are resistant to these drugs, therefore, new targets must be identified to treat drug-resistant strains. This work is focused on the structural and functional characterization of three proteins: spNadC, spNadD, and spNadE. These enzymes are involved in the biosynthesis of nicotinamide adenine dinucleotide (NAD ). The structures of spNadC and spNadE were determined. SpNadC is suggested to play a role in GAS virulence, while spNadE, functions as an NAD synthetase and is considered to be a new drug target. Determination of the spNadE structure uncovered a putative, NH channel, which may provide insight into the mechanistic details of NH -dependent NAD synthetases in prokaryotes.

PubMed: 28618168
DOI: 10.1111/febs.14136

Experimental method

X-RAY DIFFRACTION (2.1 Å)