5HOO

Crystal structure of the Mos1 Strand Transfer Complex

5HOO の概要

• エントリーDOI: 10.2210/pdb5hoo/pdb
• 関連するPDBエントリー: 3HOS 3HOT 4R79 4U7B
• 分子名称: Mariner Mos1 transposase, Mos1 IR DNA NTS, Mos1 IR TS joined to Target DNA,Mos1 IR TS joined to Target DNA, ... (6 entities in total)
• 機能のキーワード: protein-dna complex, dna transposase, recombinase, integrase, helix-turn-helix, base flipping, dna
• 由来する生物種: Drosophila mauritiana (Fruit fly); 詳細
• 細胞内の位置: Nucleus : Q7JQ07
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 125415.52

構造登録者

Richardson, J.M.,Morris, E.R. (登録日: 2016-01-19, 公開日: 2016-06-01, 最終更新日: 2024-11-06)

主引用文献

Morris, E.R.,Grey, H.,McKenzie, G.,Jones, A.C.,Richardson, J.M.
A bend, flip and trap mechanism for transposon integration.
Elife, 5:-, 2016

PubMed Abstract: Cut-and-paste DNA transposons of the mariner/Tc1 family are useful tools for genome engineering and are inserted specifically at TA target sites. A crystal structure of the mariner transposase Mos1 (derived from Drosophila mauritiana), in complex with transposon ends covalently joined to target DNA, portrays the transposition machinery after DNA integration. It reveals severe distortion of target DNA and flipping of the target adenines into extra-helical positions. Fluorescence experiments confirm dynamic base flipping in solution. Transposase residues W159, R186, F187 and K190 stabilise the target DNA distortions and are required for efficient transposon integration and transposition in vitro. Transposase recognises the flipped target adenines via base-specific interactions with backbone atoms, offering a molecular basis for TA target sequence selection. Our results will provide a template for re-designing mariner/Tc1 transposases with modified target specificities.

PubMed: 27223327
DOI: 10.7554/eLife.15537

実験手法

X-RAY DIFFRACTION (3.3 Å)