5HIP

Crystal Structure of PQS Response Protein PqsE in Complex with 2-(pyridin-3-yl)benzoic acid

5HIP の概要

• エントリーDOI: 10.2210/pdb5hip/pdb
• 分子名称: PqsE, FE (III) ION, 2-(pyridin-3-yl)benzoic acid, ... (4 entities in total)
• 機能のキーワード: quorum sensing, pqs, pqse, inhibitor, pseudomonas, metal binding protein
• 由来する生物種: Pseudomonas aeruginosa PAO1
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 34856.39

構造登録者

Witzgall, F.,Blankenfeldt, W. (登録日: 2016-01-12, 公開日: 2016-04-27, 最終更新日: 2024-10-23)

主引用文献

Zender, M.,Witzgall, F.,Drees, S.L.,Weidel, E.,Maurer, C.K.,Fetzner, S.,Blankenfeldt, W.,Empting, M.,Hartmann, R.W.
Dissecting the Multiple Roles of PqsE in Pseudomonas aeruginosa Virulence by Discovery of Small Tool Compounds.
Acs Chem.Biol., 11:1755-1763, 2016

PubMed Abstract: Pseudomonas aeruginosa uses quorum sensing (QS) as a cell-to-cell communication system to orchestrate the expression of virulence determinants. The biosynthesis of the important Pseudomonas quinolone signal (PQS) requires the pqsABCDE operon. Here, PqsE acts as a pathway-specific thioesterase, but it also contributes to the regulation of bacterial virulence via an unknown mechanism. In this manuscript, we report the discovery of PqsE inhibitors as tool compounds to gain further insights into its different functions. Differential scanning fluorimetry (DSF) was used to screen a fragment library, and isothermal titration calorimetry (ITC) was employed as a secondary filter. As proven by X-ray crystallography, hit molecules bound to the active center inhibiting PqsE's thioesterase activity in cell-based and in vitro assays. Notably, the ligands did not affect the levels of the PqsE-regulated virulence factor pyocyanin. These findings indicate that the regulatory function of PqsE is not linked to its thioesterase activity and must be encoded outside of the active center. This study highlights the potential of fragment-based screening for the discovery of tool compounds. This approach provided novel insight into complex biological systems, which could not be obtained by knockout studies.

PubMed: 27082157
DOI: 10.1021/acschembio.6b00156

実験手法

X-RAY DIFFRACTION (1.992 Å)