5HDJ

Structure of B. megaterium NfrA1

5HDJ の概要

• エントリーDOI: 10.2210/pdb5hdj/pdb
• 分子名称: NfrA1, FLAVIN MONONUCLEOTIDE, 1,2-ETHANEDIOL, ... (4 entities in total)
• 機能のキーワード: nitroreductase enzyme, dimer, oxidoreductase
• 由来する生物種: Bacillus megaterium
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 59791.30

構造登録者

Vigouroux, A.,Morera, S. (登録日: 2016-01-05, 公開日: 2016-04-06, 最終更新日: 2024-01-10)

主引用文献

Carles, L.,Besse-Hoggan, P.,Joly, M.,Vigouroux, A.,Morera, S.,Batisson, I.
Functional and structural characterization of two Bacillus megaterium nitroreductases biotransforming the herbicide mesotrione.
Biochem.J., 473:1443-1453, 2016

PubMed Abstract: Mesotrione is a selective herbicide belonging to the triketone family, commonly used on maize cultures since 2003. A mesotrione-transforming Bacillus megaterium Mes11 strain isolated from an agricultural soil was used as a model to identify the key enzymes initiating the biotransformation of this herbicide. Two enzymes (called NfrA1 and NfrA2/YcnD) were identified, and functionally and structurally characterized. Both belong to the NfsA FRP family of the nitro-FMN reductase superfamily (type I oxygen-insensitive nitroreductase) and show optimal pH and temperature of 6-6.5 and 23-25°C, respectively. Both undergo a Ping Pong Bi Bi mechanism, with NADPH and NADPH/NADH as cofactors for NfrA1 and NfrA2/YcnD, respectively. It is interesting that both can also reduce various nitro compounds including pesticides, antibiotics, one prodrug and 4-methylsulfonyl-2-nitrobenzoic acid, one of the mesotrione metabolites retrieved from the environment. The present study constitutes the first identification of mesotrione-transforming enzymes. These enzymes (or their corresponding genes) could be used as biomarkers to predict the capacity of ecosystems to transform mesotrione and assess their contamination by both the parent molecule and/or the metabolites.

PubMed: 27005432
DOI: 10.1042/BJ20151366

実験手法

X-RAY DIFFRACTION (1.89 Å)