5HDD

Femtosecond Structural Dynamics Drives the Trans/Cis Isomerization in Photoactive Yellow Protein: 800 fs to 1200 fs Structure

5HDD の概要

• エントリーDOI: 10.2210/pdb5hdd/pdb
• 関連するPDBエントリー: 2PHY 5HD3 5HD5 5HDC 5HDS
• 分子名称: Photoactive yellow protein (2 entities in total)
• 機能のキーワード: photoreceptor cis trans isomerization trans cis isomerization free electron laser, signaling protein
• 由来する生物種: Halorhodospira halophila
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14034.72

構造登録者

Pande, K.,Tenboer, J.,Schmidt, M. (登録日: 2016-01-05, 公開日: 2016-05-25, 最終更新日: 2023-11-15)

主引用文献

Pande, K.,Hutchison, C.D.,Groenhof, G.,Aquila, A.,Robinson, J.S.,Tenboer, J.,Basu, S.,Boutet, S.,DePonte, D.P.,Liang, M.,White, T.A.,Zatsepin, N.A.,Yefanov, O.,Morozov, D.,Oberthuer, D.,Gati, C.,Subramanian, G.,James, D.,Zhao, Y.,Koralek, J.,Brayshaw, J.,Kupitz, C.,Conrad, C.,Roy-Chowdhury, S.,Coe, J.D.,Metz, M.,Xavier, P.L.,Grant, T.D.,Koglin, J.E.,Ketawala, G.,Fromme, R.,Srajer, V.,Henning, R.,Spence, J.C.,Ourmazd, A.,Schwander, P.,Weierstall, U.,Frank, M.,Fromme, P.,Barty, A.,Chapman, H.N.,Moffat, K.,van Thor, J.J.,Schmidt, M.
Femtosecond structural dynamics drives the trans/cis isomerization in photoactive yellow protein.
Science, 352:725-729, 2016

PubMed Abstract: A variety of organisms have evolved mechanisms to detect and respond to light, in which the response is mediated by protein structural changes after photon absorption. The initial step is often the photoisomerization of a conjugated chromophore. Isomerization occurs on ultrafast time scales and is substantially influenced by the chromophore environment. Here we identify structural changes associated with the earliest steps in the trans-to-cis isomerization of the chromophore in photoactive yellow protein. Femtosecond hard x-ray pulses emitted by the Linac Coherent Light Source were used to conduct time-resolved serial femtosecond crystallography on photoactive yellow protein microcrystals over a time range from 100 femtoseconds to 3 picoseconds to determine the structural dynamics of the photoisomerization reaction.

PubMed: 27151871
DOI: 10.1126/science.aad5081

実験手法

X-RAY DIFFRACTION (1.6 Å)