5HD5

Femtosecond Structural Dynamics Drives the Trans/Cis Isomerization in Photoactive Yellow Protein: 200 ns time delay photo-activated (light) structure

Summary for 5HD5

• Entry DOI: 10.2210/pdb5hd5/pdb
• Related: 2PHY 5HD3 5HDC 5HDD 5HDS
• Descriptor: Photoactive yellow protein (2 entities in total)
• Functional Keywords: photoreceptor cis trans isomerization free electron laser, signaling protein
• Biological source: Halorhodospira halophila
• Total number of polymer chains: 1
• Total formula weight: 14034.72

Authors

Pande, K.,Tenboer, J.,Schmidt, M. (deposition date: 2016-01-04, release date: 2016-05-25, Last modification date: 2023-11-15)

Primary citation

Pande, K.,Hutchison, C.D.,Groenhof, G.,Aquila, A.,Robinson, J.S.,Tenboer, J.,Basu, S.,Boutet, S.,DePonte, D.P.,Liang, M.,White, T.A.,Zatsepin, N.A.,Yefanov, O.,Morozov, D.,Oberthuer, D.,Gati, C.,Subramanian, G.,James, D.,Zhao, Y.,Koralek, J.,Brayshaw, J.,Kupitz, C.,Conrad, C.,Roy-Chowdhury, S.,Coe, J.D.,Metz, M.,Xavier, P.L.,Grant, T.D.,Koglin, J.E.,Ketawala, G.,Fromme, R.,Srajer, V.,Henning, R.,Spence, J.C.,Ourmazd, A.,Schwander, P.,Weierstall, U.,Frank, M.,Fromme, P.,Barty, A.,Chapman, H.N.,Moffat, K.,van Thor, J.J.,Schmidt, M.
Femtosecond structural dynamics drives the trans/cis isomerization in photoactive yellow protein.
Science, 352:725-729, 2016

PubMed Abstract: A variety of organisms have evolved mechanisms to detect and respond to light, in which the response is mediated by protein structural changes after photon absorption. The initial step is often the photoisomerization of a conjugated chromophore. Isomerization occurs on ultrafast time scales and is substantially influenced by the chromophore environment. Here we identify structural changes associated with the earliest steps in the trans-to-cis isomerization of the chromophore in photoactive yellow protein. Femtosecond hard x-ray pulses emitted by the Linac Coherent Light Source were used to conduct time-resolved serial femtosecond crystallography on photoactive yellow protein microcrystals over a time range from 100 femtoseconds to 3 picoseconds to determine the structural dynamics of the photoisomerization reaction.

PubMed: 27151871
DOI: 10.1126/science.aad5081

Experimental method

X-RAY DIFFRACTION (1.6 Å)